Lichens-Park A, Syvanen M
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Mol Gen Genet. 1988 Feb;211(2):244-51. doi: 10.1007/BF00330600.
The insertion sequence, IS50R, promotes cointegrate formation between a lambda::IS50R phage and the chromosome of Escherichia coli strain C. We show that formation of cointegrates mediated by IS50R between the non-replicating phage genome and the bacterial chromosome requires multiple donor molecules and depends on homologous recombination functions. We conclude that the two copies of IS50 present in the cointegrate originate in two different molecules. Thus, the existence of the cointegrate structure cannot be used as evidence for replication of IS50 sequences during IS50 transposition.
插入序列IS50R促进了λ::IS50R噬菌体与大肠杆菌C菌株染色体之间共合体的形成。我们发现，由IS50R介导的非复制型噬菌体基因组与细菌染色体之间共合体的形成需要多个供体分子，并且依赖于同源重组功能。我们得出结论，共合体中存在的两个IS50拷贝起源于两个不同的分子。因此，共合体结构的存在不能作为IS50转座过程中IS50序列复制的证据。
