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果蝇乙酰胆碱受体相关基因在中枢神经系统中的表达。

Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system.

作者信息

Wadsworth S C, Rosenthal L S, Kammermeyer K L, Potter M B, Nelson D J

机构信息

Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

出版信息

Mol Cell Biol. 1988 Feb;8(2):778-85. doi: 10.1128/mcb.8.2.778-785.1988.

DOI:10.1128/mcb.8.2.778-785.1988
PMID:2832736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363204/
Abstract

We isolated Drosophila melanogaster genomic sequences with nucleotide and amino acid sequence homology to subunits of vertebrate acetylcholine receptor by hybridization with a Torpedo acetylcholine receptor subunit cDNA probe. Five introns are present in the portion of the Drosophila gene encoding the unprocessed protein and are positionally conserved relative to the human acetylcholine receptor alpha-subunit gene. The Drosophila genomic clone hybridized to salivary gland polytene chromosome 3L within region 64B and was termed AChR64B. A 3-kilobase poly(A)-containing transcript complementary to the AChR64B clone was readily detectable by RNA blot hybridizations during midembryogenesis, during metamorphosis, and in newly enclosed adults. AChR64B transcripts were localized to the cellular regions of the central nervous system during embryonic, larval, pupal, and adult stages of development. During metamorphosis, a temporal relationship between the morphogenesis of the optic lobe and expression of AChR64B transcripts was observed.

摘要

我们通过用一种电鳐乙酰胆碱受体亚基cDNA探针杂交，分离出了与脊椎动物乙酰胆碱受体亚基具有核苷酸和氨基酸序列同源性的黑腹果蝇基因组序列。果蝇基因中编码未加工蛋白质的部分存在五个内含子，并且相对于人类乙酰胆碱受体α-亚基基因，它们在位置上是保守的。果蝇基因组克隆与唾液腺多线染色体3L上64B区域杂交，被命名为AChR64B。在胚胎中期、变态期间以及新羽化的成虫中，通过RNA印迹杂交很容易检测到与AChR64B克隆互补的一个3千碱基含poly(A)的转录本。在胚胎、幼虫、蛹和成虫发育阶段，AChR64B转录本定位于中枢神经系统的细胞区域。在变态期间，观察到视叶形态发生与AChR64B转录本表达之间的时间关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/b2fc0ddaa53b/molcellb00062-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/2c3ec0cd122c/molcellb00062-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/bd29b6853fa3/molcellb00062-0277-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/bc9b2026d116/molcellb00062-0277-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/02ce1c0517d6/molcellb00062-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/b2fc0ddaa53b/molcellb00062-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/2c3ec0cd122c/molcellb00062-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/bd29b6853fa3/molcellb00062-0277-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/bc9b2026d116/molcellb00062-0277-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/02ce1c0517d6/molcellb00062-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c97/363204/b2fc0ddaa53b/molcellb00062-0279-a.jpg

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