Fyrberg E A, Mahaffey J W, Bond B J, Davidson N
Cell. 1983 May;33(1):115-23. doi: 10.1016/0092-8674(83)90340-9.
We have surveyed expression of the six Drosophila actin genes during ontogeny. Unique portions of cloned actin genes were used to monitor levels of respective mRNAs in developmentally staged whole organisms and dissected body parts. We find that each gene is transcribed to form functional mRNA, which accumulates with a distinct pattern. Two of the genes, act5C and act42A, are expressed in undifferentiated cells and probably encode cytoplasmic actins. Act57A and act87E are expressed predominantly in larval, pupal, and adult intersegmental muscles; act88F in muscles of the adult thorax; and act79B in the thorax and leg muscles. These composite data define three main patterns of actin gene expression which are correlated with changing Drosophila morphology, particularly muscle differentiation and reorganization.
我们已经研究了六种果蝇肌动蛋白基因在个体发育过程中的表达情况。克隆的肌动蛋白基因的独特部分被用于监测发育阶段完整生物体和解剖身体部位中各自mRNA的水平。我们发现每个基因都转录形成功能性mRNA，且以独特的模式积累。其中两个基因，act5C和act42A，在未分化细胞中表达，可能编码细胞质肌动蛋白。Act57A和act87E主要在幼虫、蛹和成虫的节间肌肉中表达；act88F在成虫胸部肌肉中表达；act79B在胸部和腿部肌肉中表达。这些综合数据定义了肌动蛋白基因表达的三种主要模式，它们与果蝇形态的变化相关，特别是肌肉分化和重组。
