Lima Julia Teresa Ribeiro de, Gennari Solange Maria, Soares Herbert Sousa, Minervino Antonio Humberto Hamad, Malheiros Antonio Francisco, Marques Fernando Silveira, Laurenti Márcia Dalastra, Machado Rosangela Zacarias, Marcili Arlei, Labruna Marcelo Bahia, Soares Rodrigo Martins
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
Instituto de Biodiversidade e Floresta, Universidade Federal do Oeste do Pará, Santarém, PA, Brasil.
Rev Soc Bras Med Trop. 2017 Jan-Feb;50(1):61-66. doi: 10.1590/0037-8682-0377-2016.
: Leishmaniasis is endemic to the Northern, Northeastern, Central-Western, and Southeastern regions of Brazil. We aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the States of Mato Grosso and Tocantins using a serological survey conducted in May 2011.
: Serum samples were collected from 470 humans and 327 dogs living in villages of the Urubu Branco and Tapirapé Karajá indigenous reserves. The samples were analyzed for the presence of Leishmania spp. antibodies using the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) with a crude antigen (CA) and soluble antigen (SA), and Dual Path Platform (DPP®) immunoassay for canine visceral leishmaniasis.
: Of 470 human samples tested, two (0.4%) were positive using IFAT. Among 327 dog samples tested, 28 (8.6%) were positive using ELISA CA, five (1.5%) using ELISA SA, two (0.6%) using IFAT, and none using DPP® immunoassay with Leishmania infantum chagasi antigen. When Leishmania amazonensis antigen was used, 20 (6.1%) samples were positive using ELISA CA and four (1.2%) using IFAT.
: There was a low prevalence of infection in the region, and significant differences among the main serological methods used for the diagnosis of leishmaniasis. These findings indicated that the detection of Leishmania spp. requires further study and improvement.
利什曼病在巴西北部、东北部、中西部和东南部地区呈地方性流行。我们旨在通过2011年5月进行的血清学调查，评估位于马托格罗索州和托坎廷斯州的土著村庄中人类和犬类利什曼病的流行病学情况。
从乌鲁布布兰科和塔皮拉佩卡拉亚土著保护区村庄的470名人类和327只犬类中采集血清样本。使用间接荧光抗体试验（IFAT）、粗抗原（CA）和可溶性抗原（SA）的酶联免疫吸附测定（ELISA）以及犬内脏利什曼病的双路径平台（DPP®）免疫测定法，分析样本中利什曼原虫属抗体的存在情况。
在检测的470份人类样本中，使用IFAT法有两份（0.4%）呈阳性。在检测的327份犬类样本中，使用ELISA CA法有28份（8.6%）呈阳性，使用ELISA SA法有5份（1.5%）呈阳性，使用IFAT法有两份（0.6%）呈阳性，使用婴儿利什曼原虫恰加斯抗原的DPP®免疫测定法无一呈阳性。当使用亚马逊利什曼原虫抗原时，使用ELISA CA法有20份（6.1%）样本呈阳性，使用IFAT法有4份（1.2%）呈阳性。
该地区感染率较低，用于诊断利什曼病的主要血清学方法之间存在显著差异。这些发现表明，利什曼原虫属的检测需要进一步研究和改进。
