Visser E S, Ambrosio R E
Section of Molecular Biology, Veterinary Research Institute, Onderstepoort.
Onderstepoort J Vet Res. 1987 Dec;54(4):623-7.
Anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. Both methods are time-consuming and labour intensive. The use of DNA probes in an hybridization assay may simplify the diagnosis of anaplasmosis in cattle and sheep. A genomic DNA library of Anaplasma centrale was constructed in an expression vector and screened to detect clones containing A. centrale DNA. Four probes which hybridized to A. centrale and Anaplasma marginale DNA were isolated. One of these (AC-1) hybridized only to A. centrale DNA, whereas AC-2, AC-3 and AC-4 could detect DNA from both A. centrale and A. marginale. Probes AC-1 and AC-2 could detect 127 ng and 8 ng DNA respectively, while AC-3 and AC-4 detected 64 ng A. centrale DNA.
无形体病可以通过免疫技术或直接显微镜检查血液涂片来诊断。这两种方法都耗时且劳动强度大。在杂交试验中使用DNA探针可能会简化牛羊无形体病的诊断。构建了一个中央无形体的基因组DNA文库，并在表达载体中进行筛选，以检测含有中央无形体DNA的克隆。分离出了四个与中央无形体和边缘无形体DNA杂交的探针。其中一个（AC-1）仅与中央无形体DNA杂交，而AC-2、AC-3和AC-4可以检测来自中央无形体和边缘无形体的DNA。探针AC-1和AC-2分别可以检测到127纳克和8纳克的DNA，而AC-3和AC-4可以检测到64纳克的中央无形体DNA。
