1 School of Biological Sciences, Nanyang Technological University (NTU), Singapore.
2 Department of Pharmacy, National University of Singapore (NUS), Singapore.
SLAS Discov. 2017 Apr;22(4):440-446. doi: 10.1177/2472555216687964. Epub 2017 Feb 7.
Analysis of interactions between molecules is of fundamental importance in life science research. In this study, we applied weak affinity chromatography, based on high-performance liquid chromatography and mass spectrometry, as a powerful tool for direct analysis of the components of a chemical reaction mixture for their binding to a target protein. As a demonstration of the potential of this method, we analyzed the binding of the compounds of the reaction mixture to the chaperone heat shock protein 90 (Hsp90). It was possible to analyze quantitatively the binding of the components of the mixture to the target independently from each other without any preceding process such as purification. This feature has wide implications in biological sciences as crude mixtures, either natural or synthetic, can be analyzed directly for their possible binding to a target. This method could lead to savings in costs and labor through shortening chemical research project development time.
分子相互作用的分析在生命科学研究中具有重要意义。在本研究中，我们应用基于高效液相色谱和质谱的弱亲和层析作为一种强大的工具，直接分析化学反应混合物的成分与靶蛋白的结合情况。作为该方法潜力的展示，我们分析了反应混合物的化合物与伴侣热休克蛋白 90（Hsp90）的结合情况。无需任何预先的处理，如纯化，就可以独立地对混合物成分与靶标的结合情况进行定量分析。这一特点在生物科学中有广泛的应用，因为可以直接分析天然或合成的粗混合物，以研究它们与靶标的可能结合情况。这种方法可以通过缩短化学研究项目开发时间来节约成本和劳动力。
