Altavilla D, Berlinghieri M C, Focà A, Cook J A
Institute of Medical Microbiology, University of Messina, Italy.
Res Exp Med (Berl). 1988;188(1):9-17. doi: 10.1007/BF01852089.
The effect of Salmonella enteritidis endotoxin on in vitro rat neutrophil cyclo-oxygenase and lipoxygenase metabolism, phagocytic activity, superoxide (O2-) generation, and microbicidal activity was investigated. Incubation of polymorphonuclear leukocytes (PMN) with 5, 25, and 50 micrograms of endotoxin significantly enhanced synthesis of immunoreactive (i) leukotriene (LT)C4/D4 and thromboxane (Tx)B2 (P less than 0.001) as compared to control cells. Endotoxin 5 micrograms/ml produced optimal stimulation of the arachidonic acid metabolites. Calcium ionophore, A23187, significantly enhanced iLTC4/D4 and iTxB2 synthesis more than that elicited with endotoxin. Although phagocytic function was not significantly altered by endotoxin, intracellular killing of C. albicans demonstrated enhanced microbicidal activity at 5 micrograms/ml of endotoxin. Superoxide generation was significantly enhanced in neutrophils stimulated with phorbol myristate acetate (PMA). Endotoxin (5 micrograms/ml) further potentiated superoxide generation by these cells when stimulated by PMA. These findings demonstrate that endotoxin directly enhances neutrophil iLTC4/D4 and iTxB2 synthesis. The enhanced arachidonic acid metabolism elicited by endotoxin in these cells parallels increased microbicidal activity and superoxide generation.
研究了肠炎沙门氏菌内毒素对体外培养的大鼠中性粒细胞环氧化酶和脂氧化酶代谢、吞噬活性、超氧化物（O2-）生成及杀菌活性的影响。与对照细胞相比，用5、25和50微克内毒素孵育多形核白细胞（PMN）可显著增强免疫反应性（i）白三烯（LT）C4/D4和血栓素（Tx）B2的合成（P<0.001）。5微克/毫升的内毒素对花生四烯酸代谢产物产生最佳刺激。钙离子载体A23187比内毒素更显著地增强了iLTC4/D4和iTxB2的合成。虽然内毒素未显著改变吞噬功能，但在5微克/毫升内毒素时，白色念珠菌的细胞内杀伤显示出增强的杀菌活性。用佛波酯（PMA）刺激中性粒细胞时，超氧化物生成显著增强。当由PMA刺激时，内毒素（5微克/毫升）进一步增强了这些细胞的超氧化物生成。这些发现表明内毒素直接增强中性粒细胞iLTC4/D4和iTxB2的合成。内毒素在这些细胞中引起的花生四烯酸代谢增强与杀菌活性增加和超氧化物生成增加平行。
