[浙江省啮齿动物中温州病毒的基因组分析]

[Genomic analysis of Wenzhou virus in rodents from Zhejiang province].

作者信息

Li K, Lin X D, Li M H, Wang M R, Sun X Y, Zhang Y Z

机构信息

Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Institute of Disinfection and Vector Control, Wenzhou Prefecture Center for Disease Control and Prevention, Wenzhou 325000, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2017 Mar 10;38(3):384-387. doi: 10.3760/cma.j.issn.0254-6450.2017.03.022.

DOI:10.3760/cma.j.issn.0254-6450.2017.03.022

PMID:28329945

Abstract

Arenavirus is a negative single-stranded RNA virus and an important human pathogen, mainly harbored and transmitted by rodents, causing severe diseases, including hemorrhagic fever and encephalitis. Following the discovery of a novel pathogenic arenavirus (Wenzhou virus, WENV), the prevalence of WENV in local small rodents was investigated. By using RT-PCR, WENV was screened in 48 and 156 rodents sampled from Wenzhou and Longquan, respectively. Consequently, WENV was detected in 5 (10.41%) rodents sampled from Wenzhou. However, no WENV was identified in all the rodents sampled from Longquan. Genetic analysis of complete genome sequences indicated that 4 of 5 virus strains were closely related to the known Wenzhou viruses with high homology. Especially, the L and S segments of Wencheng-Rn-288 strain shared homology of 87.5% and 91.6% with other viruses, respectively. They formed a distinct lineage, suggesting that this strain might be a novel variant of WENV. Our results indicate that WENV has a high prevalence and high genetic diversity among rodents in Wenzhou. As the respiratory disease caused by WENV has been detected in Cambodia, it is necessary to strengthen the surveillance for WENV in China.

摘要

沙粒病毒是一种负链单股RNA病毒，是一种重要的人类病原体，主要由啮齿动物携带和传播，可引发包括出血热和脑炎在内的严重疾病。在发现一种新型致病性沙粒病毒（温州病毒，WENV）后，对当地小型啮齿动物中WENV的流行情况进行了调查。通过逆转录聚合酶链反应（RT-PCR），分别在从温州和龙泉采集的48只和156只啮齿动物中筛选出WENV。结果，在从温州采集的5只（10.41%）啮齿动物中检测到WENV。然而，在从龙泉采集的所有啮齿动物中均未发现WENV。对完整基因组序列的遗传分析表明，5个病毒株中的4个与已知的温州病毒密切相关，具有高度同源性。特别是，文成-Rn-288株的L和S片段与其他病毒的同源性分别为87.5%和91.6%。它们形成了一个独特的谱系，表明该毒株可能是WENV的一个新变种。我们的结果表明，WENV在温州的啮齿动物中具有高流行率和高遗传多样性。由于在柬埔寨已检测到由WENV引起的呼吸道疾病，因此有必要在中国加强对WENV的监测。