Jadhav Shrinivas S, David M
Environmental and Molecular Toxicology Laboratory, Department of PG Studies in Zoology, Karnatak University, Dharwad, Karnataka, 580003, India.
3 Biotech. 2016 Jun;6(1):31. doi: 10.1007/s13205-015-0347-9. Epub 2016 Jan 14.
Flubendiamide, as a new class (Phthalic acid diamide) of pesticide with a wide spectrum of activity against lepidopteran pests extensively used alone or in combination with other insecticides in agriculture system to get protection from insect pests. Due to high specificity and limited approach towards non-target organism, the extensive use of this pesticide as an alternate for organophosphate and organochlorine pesticides, causing an eventual increase in environmental pollution. Five flubendiamide-resistant bacterial strains were isolated during the present study from agriculture soil considering previous history of pesticide application. Minimal inhibitory concentration of all the isolates showed strain SSJ1 was most efficient flubendiamide resistant organism. Biochemical tests and molecular sequencing of 16s rRNA was carried out which confirmed the isolate as Chryseobacterium indologenes strain SSJ1. UV-visible spectrophotometer study revealed that 89.06 % initial pesticide was removed by the isolate at optimum temperature of 35 °C and pH 7.0 with 5 days incubation period and is further confirmed by high-performance liquid chromatography (HPLC) analysis. Results of the present study however, suggest strain SSJ1 is most resistant to flubendiamide and can possibly be applied in the bioremediation of flubendiamide contaminated soils.
氟苯虫酰胺作为一类新型（邻苯二甲酰胺类）杀虫剂，对鳞翅目害虫具有广泛的活性，在农业系统中广泛单独使用或与其他杀虫剂混合使用，以防治害虫。由于其高特异性以及对非靶标生物影响有限，这种杀虫剂作为有机磷和有机氯农药的替代品被广泛使用，最终导致环境污染加剧。在本研究中，考虑到先前的农药使用历史，从农业土壤中分离出了五株抗氟苯虫酰胺的细菌菌株。所有分离菌株的最低抑菌浓度表明，菌株SSJ1是最有效的抗氟苯虫酰胺生物。进行了生化试验和16s rRNA分子测序，证实该分离菌株为吲哚金黄杆菌菌株SSJ1。紫外可见分光光度计研究表明，在35℃的最佳温度、pH值7.0以及5天的培养期条件下，该分离菌株去除了89.06%的初始农药，高效液相色谱（HPLC）分析进一步证实了这一结果。然而，本研究结果表明，菌株SSJ1对氟苯虫酰胺具有最强抗性，可能可应用于氟苯虫酰胺污染土壤的生物修复。
