Verma Jay Prakash, Jaiswal Durgesh Kumar, Krishna Ram, Prakash Satya, Yadav Janardan, Singh Vijai
Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, India.
Hawkesbury Institute for the Environment, Western Sydney University, Sydney, NSW, Australia.
Front Microbiol. 2018 Jun 26;9:1293. doi: 10.3389/fmicb.2018.01293. eCollection 2018.
In the present investigation, the main aim is to identify and characterize the potential drought tolerant plant growth promoting consortium for agricultural productivity. Three bacterial isolates were isolated from hot spring of Chumathang area of Leh district. species (BHUJP-H1, BHUJP-H2, and BHUJP-H3) were done some biochemical tests including catalase, cellulase, amylase, indole-3-acetic acid, phosphate solubilisation, production of ammonia, siderophore, and hydrogen cyanide. Molecular characterization of isolates was done by 16S rDNA sequencing, e.g., BHUJP-H1 (KU312403), sp. BHUJP-H2 (KU312404) and BHUJP-H3 (KU312405). The genetic diversity of the isolates was assessed by seven inter simple sequence repeat, all primer shows high polymorphism. The highest polymorphism efficiency and polymorphism information content showed by UBC-809 and UBC-836 which were 100% and 0.44 respectively, the lowest is by UBC-807 75% and 0.28 respectively. On an average 90.69% polymorphism efficiency and 0.40 polymorphism information contents obtained by used markers. The highest, 11.08 and the lowest, 4.50 effective multiplex ratios obtained for primer UBC-823 and UBC-807, on an average 7.99 effective multiplex ratio obtained. The highest, 4.89 and the lowest, 1.25 marker indexes obtained by UBC-836 and UBC-807 respectively and on an average 3.24 obtained. The UPGMA cluster analysis divided a population into two clusters I and II, in which BHUJP-H1 and BHUJP-H2 grouped under same while BHUJP-H3 grouped under another cluster. The treatment combination of BHUJP-H1, BHUJP-H1+ BHUJP-H3 and BHUJP-H1+ sp. BHUJP-H2+ BHUJP-H3 were recorded better combination for enhancing plant growth attributes of as compared to control and others. The plant growth promoting consortium, e.g., BHUJP-H1, BHUJP-H1+ BHUJP-H3 and BHUJP-H1+ sp. BHUJP-H2+ BHUJP-H3 can be further used as effective microbial inoculant for enhancing the production of mungbean in field conditions. sp. BHUJP-H1 and sp. BHUJP-H2 may use as drought tolerant plant growth promoting consortium for enhancing the sustainable agricultural productivity.
在本研究中，主要目的是鉴定和表征具有促进植物生长潜力的耐旱菌群，以提高农业生产力。从列城楚马唐地区的温泉中分离出三株细菌菌株。对这些菌株（BHUJP-H1、BHUJP-H2和BHUJP-H3）进行了一些生化测试，包括过氧化氢酶、纤维素酶、淀粉酶、吲哚-3-乙酸、磷溶解、氨生成、铁载体和氰化氢的产生。通过16S rDNA测序对分离株进行分子表征，例如，BHUJP-H1（KU312403）、芽孢杆菌属BHUJP-H2（KU312404）和芽孢杆菌属BHUJP-H3（KU312405）。通过七个简单重复序列区间评估分离株的遗传多样性，所有引物均显示出高多态性。UBC-809和UBC-836显示出最高的多态性效率和多态性信息含量，分别为100%和0.44，最低的是UBC-807，分别为75%和0.28。所使用的标记平均获得90.69%的多态性效率和0.40的多态性信息含量。引物UBC-823获得的最高有效多重比率为11.08，UBC-807获得的最低有效多重比率为4.50，平均有效多重比率为7.99。UBC-836获得的最高标记指数为4.89，UBC-807获得的最低标记指数为1.25，平均为3.24。UPGMA聚类分析将群体分为两个聚类I和II，其中BHUJP-H1和BHUJP-H2归为同一聚类，而BHUJP-H3归为另一聚类。与对照和其他处理相比，BHUJP-H1、BHUJP-H1 + BHUJP-H3以及BHUJP-H1 + 芽孢杆菌属BHUJP-H2 + BHUJP-H3的处理组合在增强绿豆植物生长特性方面表现出更好的组合效果。植物生长促进菌群，例如BHUJP-H1、BHUJP-H1 + BHUJP-H3以及BHUJP-H1 + 芽孢杆菌属BHUJP-H2 + BHUJP-H3可进一步用作有效的微生物接种剂，以提高田间条件下绿豆的产量。芽孢杆菌属BHUJP-H1和芽孢杆菌属BHUJP-H2可作为耐旱植物生长促进菌群，以提高可持续农业生产力。
