Zhang Ying-Hua, Xu Di, Zhao Xin-Huai, Song Yao, Liu Yan-Le, Li Hong-Nan
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Department of Food Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
3 Biotech. 2016 Jun;6(1):73. doi: 10.1007/s13205-016-0364-3. Epub 2016 Feb 16.
Biodegradation of the pesticides is considered as one of the safest and cheapest ways. The aim of the present study was to reveal if the inoculated Lactic acid bacteria widely used in silage could provide beneficial safety to guarantee dairy feedstuffs and fresh milk. Organophosphorus pesticides (OPPs) degradation in whole corn silage was investigated. Two OPPs, chlorpyrifos and phorate, were added to the whole corn, which was inoculated with L. plantarum 1.0315, L. plantarum 1.0624, L. plantarum 1.0622 and their combination at room temperature for 10 weeks. OPPs residues in the whole corn silage at different weeks were analyzed by gas chromatography after OPPs extraction and purification. The degradation rate constants were calculated according to the OPPs residues results at different fermentation stage. The data showed that the inoculated microorganisms and strain combination exhibited obvious acceleration on OPPs degradation as the wild microorganisms did, and resulted in decreased levels of OPPs from 24.9 to 33.4 %. Strains combination brought about greater OPPs degradation than single strain or the wild microorganisms. Compared to phorate, clorpyrifos had lower degradation rate constants (0.0274-0.0381 vs. 0.0295-0.0355 week) and was more stable. The present result indicates safety benefit of lactic acid bacteria on corn silage besides lactic acid fermentation.
农药的生物降解被认为是最安全、最便宜的方法之一。本研究的目的是揭示青贮饲料中广泛使用的接种乳酸菌是否能提供有益的安全性，以确保奶牛饲料和生鲜牛奶的安全。研究了全株玉米青贮饲料中有机磷农药（OPPs）的降解情况。将两种OPPs，毒死蜱和甲拌磷，添加到全株玉米中，在室温下接种植物乳杆菌1.0315、植物乳杆菌1.0624、植物乳杆菌1.0622及其组合，培养10周。在OPPs提取和纯化后，通过气相色谱法分析不同周龄全株玉米青贮饲料中的OPPs残留量。根据不同发酵阶段的OPPs残留结果计算降解速率常数。数据表明，接种的微生物和菌株组合对OPPs降解表现出明显的促进作用，与野生微生物一样，使OPPs水平降低了24.9%至33.4%。菌株组合比单一菌株或野生微生物带来更大的OPPs降解。与甲拌磷相比，毒死蜱的降解速率常数较低（0.0274 - 0.0381对0.0295 - 0.0355周），且更稳定。目前的结果表明，除了乳酸发酵外，乳酸菌对玉米青贮饲料还有安全益处。
