Malhotra Nikhil, Sood Hemant, Chauhan Rajinder Singh
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, 173234, India.
3 Biotech. 2016 Dec;6(2):152. doi: 10.1007/s13205-016-0466-y. Epub 2016 Jul 11.
Tuberous roots of Aconitum heterophyllum constitute storage organ for secondary metabolites, however, molecular components contributing to their formation are not known. The transcriptomes of A. heterophyllum were analyzed to identify possible genes associated with tuberous root development by taking clues from genes implicated in other plant species. Out of 18 genes, eight genes encoding GDP-mannose pyrophosphorylase (GMPase), SHAGGY, Expansin, RING-box protein 1 (RBX1), SRF receptor kinase (SRF), β-amylase, ADP-glucose pyrophosphorylase (AGPase) and Auxin responsive factor 2 (ARF2) showed higher transcript abundance in roots (13-171 folds) compared to shoots. Comparative expression analysis of those genes between tuberous root developmental stages showed 11-97 folds increase in transcripts in fully developed roots compared to young rootlets, thereby implying their association in biosynthesis, accumulation and storage of primary metabolites towards root biomass. Cluster analysis revealed a positive correlation with the gene expression data for different stages of tuberous root formation in A. heterophyllum. The outcome of this study can be useful in genetic improvement of A. heterophyllum for root biomass yield.
异叶乌头的块根是次生代谢产物的储存器官，然而，参与其形成的分子成分尚不清楚。通过借鉴其他植物物种中涉及的基因线索，分析了异叶乌头的转录组，以鉴定与块根发育相关的可能基因。在18个基因中，编码GDP-甘露糖焦磷酸化酶（GMPase）、SHAGGY、扩展蛋白、环盒蛋白1（RBX1）、SRF受体激酶（SRF）、β-淀粉酶、ADP-葡萄糖焦磷酸化酶（AGPase）和生长素响应因子2（ARF2）的8个基因在根中的转录丰度比茎高（13-171倍）。对这些基因在块根发育阶段之间的比较表达分析表明，与幼嫩根相比，完全发育的根中转录本增加了11-97倍，这意味着它们与根生物量中初级代谢产物的生物合成、积累和储存有关。聚类分析揭示了与异叶乌头块根形成不同阶段的基因表达数据呈正相关。本研究结果可用于异叶乌头根生物量产量的遗传改良。
