特定微生物群落对氯酚的多底物生物降解
Multi-substrate biodegradation of chlorophenols by defined microbial consortium.
作者信息
Patel Bhishma P, Kumar Arvind
机构信息
Value Addition, Research and Development Department, National Innovation Foundation-India, Satellite Complex, Jodhpur Tekra, Ahmedabad, 380015, Gujarat, India.
Environmental Pollution Abatement Lab, Chemical Engineering Department, National Institute of Technology, Rourkela, 769008, Odisha, India.
出版信息
3 Biotech. 2016 Dec;6(2):191. doi: 10.1007/s13205-016-0511-x. Epub 2016 Sep 2.
In the present study, a defined mixed microbial consortium was investigated for their ability to utilize three different monochlorophenols (MCPs) and 2,4-DCP individually and in the mixture. None of the individual strains were able to utilize 3-CP and 4-CP, but when they were mixed to form defined consortium, they have shown great potential and degradation of high concentration of 3-CP and 4-CP. Spectrophotometric analysis of metabolites during MCPs degradation establishes the presence of 2-chloromaleylacetate. Multi-substrate degradation study of 2,4-DCP in the presence of three MCPs showed the great prospect of microbial consortium for in situ bioremediation. During multi-substrate degradation, the biodegradation rate (mg L day) was observed in the order of 2,4-DCP > 2CP > 3CP > 4CP. Biodegradation kinetic of three MCPs using Andrew's model showed maximum removal rate (R ) of 2.78, 0.91, 1.82 mg L h for 2-CP, 3-CP and 4-CP, respectively.
在本研究中，对一个特定的混合微生物群落利用三种不同单氯酚（MCPs）以及单独和混合状态下的2,4-二氯酚（2,4-DCP）的能力进行了研究。没有任何单一菌株能够利用3-氯酚（3-CP）和4-氯酚（4-CP），但当它们混合形成特定群落时，显示出了对高浓度3-CP和4-CP的巨大降解潜力。MCPs降解过程中代谢产物的分光光度分析确定了2-氯马来酰乙酸的存在。在三种MCPs存在的情况下对2,4-DCP进行的多底物降解研究表明，微生物群落用于原位生物修复具有广阔前景。在多底物降解过程中，观察到的生物降解速率（mg/L·天）顺序为2,4-DCP＞2-氯酚（2-CP）＞3-CP＞4-CP。使用Andrew模型对三种MCPs进行的生物降解动力学研究表明，2-CP、3-CP和4-CP的最大去除率（R）分别为2.78、0.91、1.82 mg/L·小时。
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