De Indranil, Gupta Sarika
Department of Biotechnology, Dr. B. Lal Institute of Biotechnology, Malviya Nagar, Jaipur, 302017, Rajasthan, India.
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Tonk, 304022, Rajasthan, India.
3 Biotech. 2016 Dec;6(2):193. doi: 10.1007/s13205-016-0509-4. Epub 2016 Sep 6.
Oil spillage sites primarily contain various types of hydrocarbons, such as linear chain, polycyclic, and aromatic compounds, posing several detrimental effects on plants. Results from our previous study showed an alteration of various metabolomic parameters, indirectly resulting in an observable decline of growth in the mung seedlings upon incubation with phenol, toluene, xylene, and hexane. This study evaluates the role of these compounds upon plant growth and focusses to mitigate the effect of the same, using some isolated plant synergistic bacteria. We isolated Proteus sp., Streptococcus sp., and Enterococcus sp., and tested the synergism of them in mung seedlings (Vigna radiata) by hydroponics. Treatment with the above-mentioned compounds significantly reduced the root and shoot length of the seedlings when compared to the control. The bacterial treatment helped in reducing the adversity due to the xenobiotic insult, by improving the root shoot length of the treated seedlings. Proteus sp. was found to be the most promising among other isolates. In another experiment, plasmid profiling of the bacterial isolates was done, yielding a band of 4.5 kb common for all, serving as a clue to be the most probable plasmid responsible for the degradation of the compounds. Results from this study clearly indicate that Proteus sp. can be explored further for its plant synergism and xenobiotic degradative capability to exploit its potential in oil spillage land reclamation and establishing vegetation.
石油泄漏地点主要含有各种类型的碳氢化合物，如直链、多环和芳香族化合物，对植物造成多种有害影响。我们之前的研究结果表明，各种代谢组学参数发生了变化，这间接导致绿豆幼苗在与苯酚、甲苯、二甲苯和己烷一起培养时，生长明显下降。本研究评估了这些化合物对植物生长的作用，并着重利用一些分离出的植物协同细菌减轻其影响。我们分离出了变形杆菌属、链球菌属和肠球菌属，并通过水培法测试了它们在绿豆幼苗（豇豆）中的协同作用。与对照相比，用上述化合物处理显著降低了幼苗的根长和茎长。细菌处理通过改善处理后幼苗的根长和茎长，有助于减轻由于异源物质侵害造成的逆境。在其他分离菌株中，变形杆菌属被发现最有前景。在另一个实验中，对细菌分离株进行了质粒图谱分析，所有菌株都产生了一条4.5 kb的条带，这是最有可能负责化合物降解的质粒的线索。这项研究的结果清楚地表明，变形杆菌属因其植物协同作用和异源物质降解能力，可以进一步探索其在石油泄漏土地复垦和植被建立中的潜力。
