Egutkin G G
Vopr Med Khim. 1987 Nov-Dec;33(6):132-5.
A rapid procedure has been described for preparation of a relatively pure fraction of plasmatic membranes from adipose tissue without treatment with collagenase. Gentle homogenization of rat fatty tissue in a buffered sucrose solution yielded membrane fractions that could be separated from the bulk of contaminating mitochondria and microsomes by a series of differential and isopycnic centrifugations. The preparation obtained was enriched 12-fold with 5'-nucleotidase as compared with the initial homogenate and contained minimal contaminations with mitochondria or elements of endoplasmic reticulum.
已描述了一种无需用胶原酶处理即可从脂肪组织制备相对纯净的质膜部分的快速方法。在缓冲蔗糖溶液中对大鼠脂肪组织进行温和匀浆，得到的膜部分可通过一系列差速离心和等密度离心与大部分污染的线粒体和微粒体分离。与初始匀浆相比，所获得的制剂中5'-核苷酸酶富集了12倍，且线粒体或内质网成分的污染极少。
