Huggins J W, Carraway K L
J Supramol Struct. 1976;5(1):59-63. doi: 10.1002/jss.400050106.
A highly purified plasma membrane fraction was obtained from a microsomal subfraction of rat mammary gland after treatment with digitonin to increase its density. The purified membranes were enriched 70-fold overall in 5'-nucleotidase with essentially no contamination from glactosyltransferase, succinate-ING reductase, or NADPH-cytochrome c reductase. The membranes were also highly enriched in sialoglycoproteins.
在用洋地黄皂苷处理以增加其密度后,从大鼠乳腺的微粒体亚组分中获得了高度纯化的质膜组分。纯化后的膜中5'-核苷酸酶总体富集了70倍,基本上没有受到半乳糖基转移酶、琥珀酸-ING还原酶或NADPH-细胞色素c还原酶的污染。这些膜中唾液糖蛋白也高度富集。