Ben Ya'acov Ami, Meir Hadar, Zolotaryova Lydia, Ilan Yaron, Shteyer Eyal
Liver Unit, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.
Department of Pediatrics, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.
BMC Gastroenterol. 2017 Mar 23;17(1):44. doi: 10.1186/s12876-017-0600-2.
It has been shown that the proportion of natural killer T cells is markedly elevated during liver regeneration and their activation under different conditions can modulate this process. As natural killer T cells and liver injury are central in liver regeneration, elucidating their role is important.
The aim of the current study is to explore the role of natural killer T cells in impaired liver regeneration. Concanvalin A was injected 4 days before partial hepatectomy to natural killer T cells- deficient mice or to anti CD1d1-treated mice. Ki-67 and proliferating cell nuclear antigen were used to measure hepatocytes proliferation. Expression of hepatic cyclin B1 and proliferating cell nuclear antigen were evaluated by Western Blot and liver injury was assessed by ALT and histology.
Natural killer T cells- deficient or mice injected with anti CD1d antibodies exhibited reduced liver regeneration. These mice were considerably resistant to ConA-induced liver injury. In the absence of NKT cells hepatic proliferating cell nuclear antigen and cyclin B1 decreased in mice injected with Concanvalin A before partial hepatectomy. This was accompanied with reduced serum interleukin-6 levels.
Natural killer T cells play an important role in liver regeneration, which is associated with cyclin B1 and interleukin-6.
研究表明，自然杀伤T细胞的比例在肝脏再生过程中显著升高，并且它们在不同条件下的激活能够调节这一过程。由于自然杀伤T细胞和肝损伤在肝脏再生中起核心作用，阐明它们的作用很重要。
本研究的目的是探讨自然杀伤T细胞在肝脏再生受损中的作用。在部分肝切除术前4天，向自然杀伤T细胞缺陷小鼠或抗CD1d1处理的小鼠注射刀豆蛋白A。使用Ki-67和增殖细胞核抗原测量肝细胞增殖。通过蛋白质免疫印迹法评估肝细胞周期蛋白B1和增殖细胞核抗原的表达，并通过谷丙转氨酶和组织学评估肝损伤。
自然杀伤T细胞缺陷小鼠或注射抗CD1d抗体的小鼠肝脏再生减少。这些小鼠对刀豆蛋白A诱导的肝损伤具有相当的抵抗力。在部分肝切除术前注射刀豆蛋白A的小鼠中，在没有自然杀伤T细胞的情况下，肝增殖细胞核抗原和细胞周期蛋白B1减少。这伴随着血清白细胞介素-6水平的降低。
自然杀伤T细胞在肝脏再生中起重要作用，这与细胞周期蛋白B1和白细胞介素-6有关。
