Gadsby J E, Tyson Nipper A M, Faircloth H A, D'Annibale-Tolhurst M, Chang J, Farin P W, Sheldon I M, Poole D H
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Department of Animal Science, North Carolina State University, Raleigh, NC, USA.
Reprod Domest Anim. 2017 Jun;52(3):495-504. doi: 10.1111/rda.12940. Epub 2017 Mar 22.
Improving our understanding of the mechanisms controlling the corpus luteum (CL) and its role in regulating the reproductive cycle should lead to improvements in the sustainability of today's global animal industry. The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture steroidogenic, endothelial and immune cells, and it is becoming clear that immune mechanisms play a key role in CL regulation especially in luteolysis. Toll-like receptors (TLR) mediate innate immune mechanisms via the production of pro-inflammatory cytokines, especially within various tissues, although the role of TLR within CL remains unknown. Thus, the objectives of this study were to characterize TLR mRNA expression in the CL during the oestrous cycle and in pregnancy (day 30-50), and to examine the role of TLR signalling in luteal cells. Corpora lutea were collected at various stages of the cycle and pregnancy and analysed for TLR and cytokine mRNA expression. In addition, luteal cells were cultured with the TLR4 ligand (lipopolysaccharide, LPS) for 24 h to evaluate the role of TLR4 in regulating luteal function. Toll-like receptors 1, 2, 4, 6, tumour necrosis factor alpha (TNF), interferon gamma (IFN-G), and interleukin (IL)-12, mRNA expressions were greatest in regressing CL compared with earlier stages (p < .05), whereas no change was observed for IL-6 mRNA expression. Cytokine mRNA expression in cultured luteal cells was not altered by LPS. Based on these data, one or more of the TLRs found within the CL may play a role in luteolysis, perhaps via pro-inflammatory cytokine mRNA expression.
增进我们对控制黄体（CL）的机制及其在调节生殖周期中作用的理解，应该会改善当今全球畜牧业的可持续性。黄体（CL）是一个短暂的内分泌器官，由类固醇生成细胞、内皮细胞和免疫细胞的异质混合物组成，并且越来越清楚的是，免疫机制在CL调节中起着关键作用，尤其是在黄体溶解过程中。Toll样受体（TLR）通过促炎细胞因子的产生介导先天免疫机制，特别是在各种组织中，尽管TLR在CL中的作用仍然未知。因此，本研究的目的是表征发情周期和妊娠期间（第30 - 50天）CL中TLR mRNA的表达，并研究TLR信号在黄体细胞中的作用。在周期和妊娠的不同阶段收集黄体，并分析TLR和细胞因子mRNA的表达。此外，将黄体细胞与TLR4配体（脂多糖，LPS）培养24小时，以评估TLR4在调节黄体功能中的作用。与早期阶段相比，在退化的CL中，Toll样受体1、2、4、6、肿瘤坏死因子α（TNF）、干扰素γ（IFN - G）和白细胞介素（IL）-12的mRNA表达最高（p < 0.05），而IL - 6 mRNA表达未观察到变化。LPS未改变培养的黄体细胞中细胞因子mRNA的表达。基于这些数据，CL中发现的一种或多种TLR可能在黄体溶解中起作用，可能是通过促炎细胞因子mRNA的表达。
