Fontinele Renata Gabriel, Krause Neto Walter, Gama Eliane Florencio, Brito Mari Renata de, de Souza Romeu Rodrigues, Conrado André, Mochizuki Luis, Kfoury Junior José Roberto
a Department of Surgery, Faculty of Veterinary Medicine and Zootecny , São Paulo University , São Paulo , Brazil.
b Department of Physical Education, Laboratory of Morphoquantitative Studies and Immunohistochemistry , São Judas Tadeu University , São Paulo , Brazil.
Aging Male. 2017 Sep;20(3):161-167. doi: 10.1080/13685538.2017.1301418. Epub 2017 Mar 23.
This study aimed to analyze the effects of caloric restriction on aged femoral articular cartilage of Wistar rats. Three groups of eight animals each were considered: young (YC) and old (OC) control groups fed with a normal diet and old caloric restriction group (OCR) composed of 18-month-old animals fed with a 31% less caloric diet from 6-months of age. Articular cartilage was studied through morphometry and immunohistochemistry. Body mass was 12% less in the OCR group than in the OC group. The articular cartilage from OC rats show thinner medial condyles, fewer chondrocytes, smaller chondrocytes nuclear volume and, in both condyles, a predominance of collagen type II and less collagen density compared to both YC and OCR groups (p < .001). In contrast, OCR articular cartilage show thicker medial condyles, larger chondrocytes nuclear volume and increased collagen density compared to OC group (p < 0.001). We concluded that caloric restriction minimizes the effects of aging on medial condyles of the femoral articular cartilage.
本研究旨在分析热量限制对Wistar大鼠老年股骨关节软骨的影响。实验分为三组，每组八只动物：年轻对照组（YC）和老年对照组（OC）给予正常饮食，老年热量限制组（OCR）由18月龄动物组成，从6月龄开始给予热量减少31%的饮食。通过形态计量学和免疫组织化学研究关节软骨。OCR组的体重比OC组轻12%。与YC组和OCR组相比，OC大鼠的关节软骨内侧髁更薄，软骨细胞更少，软骨细胞核体积更小，并且在两个髁中，II型胶原占优势，胶原密度更低（p < 0.001）。相反，与OC组相比，OCR组的关节软骨内侧髁更厚，软骨细胞核体积更大，胶原密度增加（p < 0.001）。我们得出结论，热量限制可将衰老对股骨关节软骨内侧髁的影响降至最低。
