School of Dentistry, Uninove University, São Paulo, Brazil.
Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil.
Acta Histochem. 2020 Feb;122(2):151501. doi: 10.1016/j.acthis.2019.151501. Epub 2019 Dec 28.
This study investigated the effects of aging on the articular cartilage of the mandible. Wistar rats were divided in two groups (n = 10/per group): 3-months-old group (young group); and 13-months-old group (aged group). After euthanasia, the head of the mandible was collected and stained with hematoxylin and eosin (HE) to evaluate the thickness of the articular layer and cartilage. Sections stained with Picrosirius red and Safranin O were used to evaluate the collagen and proteoglycans deposition, respectively. First, aging has decreased the articular layer thickness. Second, the results suggest a decrease of chondrocytes followed by an increase of the matrix to maintain the mandible homeostasis. Finally, both collagen and proteoglycans increased with aging. Aging displayed important effects to the mandible of aged rats.
本研究调查了年龄对下颌骨关节软骨的影响。Wistar 大鼠分为两组（每组 n=10）：3 月龄组（年轻组）；和 13 月龄组（老年组）。安乐死后，采集下颌骨头部，用苏木精和伊红（HE）染色，评估关节层和软骨的厚度。用番红 O 和苦味酸天狼星红染色的切片分别用于评估胶原蛋白和蛋白聚糖的沉积。首先，衰老减少了关节层的厚度。其次，结果表明软骨细胞减少，随后基质增加，以维持下颌骨的内稳态。最后，胶原蛋白和蛋白聚糖随年龄增长而增加。衰老对老年大鼠的下颌骨有重要影响。
