大鼠体内钙激活中性蛋白酶内源性抑制剂的组织分布及其活性的年龄相关变化

Tissue distribution of an endogenous inhibitor of calcium-activated neutral protease and age-related changes in its activity in rats.

Nakamura M, Imahori K, Kawashima S

Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.

Comp Biochem Physiol B. 1988;89(2):381-4. doi: 10.1016/0305-0491(88)90240-4.

Abstract

The distribution of an endogenous inhibitor of calcium-activated neutral protease (CANP) and age-related changes in its activity were studied in male and female rats of different ages by a fluorometric assay on tissue extracts after heat treatment. 2. Ubiquitous distribution of CANP inhibitor in brain, cardiac muscle, lung, spleen, liver, skeletal muscle, kidney and testis and its abundance in spleen, liver and kidney was demonstrated. 3. Comparison in terms of units/ml of crude extracts showed that the level of CANP inhibitor exceeded that of CANP in most tissues and that the relative content of CANP inhibitor to mCANP and microCANP differed greatly among tissues. 4. Sex and species differences in CANP inhibitor activity in each tissue were of little significance. 5. Changes in CANP inhibitor during aging from 6 to 12 months was not obvious but senescent rats showed a tendency toward increased inhibitor activity. This increase was especially evident in the testis.

摘要

通过对热处理后的组织提取物进行荧光测定，研究了钙激活中性蛋白酶（CANP）内源性抑制剂在不同年龄雄性和雌性大鼠中的分布及其与年龄相关的活性变化。2. 证实了CANP抑制剂在脑、心肌、肺、脾、肝、骨骼肌、肾和睾丸中普遍存在，且在脾、肝和肾中含量丰富。3. 粗提取物单位/毫升的比较表明，大多数组织中CANP抑制剂的水平超过了CANP，并且CANP抑制剂与mCANP和微CANP的相对含量在不同组织中差异很大。4. 各组织中CANP抑制剂活性的性别和物种差异不太显著。5. 6至12个月龄衰老过程中CANP抑制剂的变化不明显，但衰老大鼠显示出抑制剂活性增加的趋势。这种增加在睾丸中尤为明显。