Kawashima S, Hayashi M, Saito Y, Kasai Y, Imahori K
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Biochim Biophys Acta. 1988 May 12;965(2-3):130-5. doi: 10.1016/0304-4165(88)90048-7.
A simple separation method involving a minimum number of essential steps was established to separate the two kinds of calcium-activated neutral proteinase (CANP) activity from each other and from endogenous CANP inhibitor activity. Determination of CANP activity in rat tissues using this method revealed a ubiquitous distribution of two CANPs. The level of CANP activity, however, differs between tissues. Low-calcium-requiring CANP (microCANP) is plentiful in spleen and kidney, while high-calcium-requiring CANP (mCANP) is plentiful in lung and brain. In all of the tissues examined, mCANP activity predominates over microCANP activity.
建立了一种包含最少必要步骤的简单分离方法，以将两种钙激活中性蛋白酶（CANP）活性彼此分离，并与内源性CANP抑制剂活性分离。使用该方法测定大鼠组织中的CANP活性，结果显示两种CANP广泛分布。然而，不同组织中的CANP活性水平有所不同。低钙需求的CANP（微CANP）在脾脏和肾脏中含量丰富，而高钙需求的CANP（mCANP）在肺和脑中含量丰富。在所有检测的组织中，mCANP活性均高于微CANP活性。
