Canon J L, Humblet Y, Lebacq-Verheyden A M, Manouvriez P, Bazin H, Rodhain J, Prignot J, Symann M
Ludwig Institute for Cancer Research, Brussels Branch, Belgium.
Eur J Cancer Clin Oncol. 1988 Feb;24(2):147-50. doi: 10.1016/0277-5379(88)90245-3.
Detection of bone marrow metastases by indirect immunofluorescence methods was investigated using three monoclonal antibodies (MoAbs) raised against small cell lung cancer (SCLC). These antibodies, designated anti-LCA1, -LCA2 and -LCA3, recognize three different antigens on the surface of SCLC cells. Eighty-four bone marrow samples from 74 different patients were studied. Whereas tumor cells were found in 32 (38%) by MoAb staining, only 10 (12%) were positively identified using conventional morphological methods. Nine out of the morphologically positive specimens showed reactivity with at least two monoclonal antibodies. Among the 32 samples proven positive by immunofluorescence, an important antigenic variability was noted. Anti-LCA1 recognized tumor cells in 62%, anti-LCA2 and anti-LCA3 in 53%. Due to the recognition of bone marrow involvement by fluorescence methods in 26% of the 34 patients classified as limited disease, a new subgroup of limited disease patients was defined whose prognosis remains undetermined. Our results confirm the utility of immunodetection in the diagnosis of SCLC bone marrow metastases and emphasize the advantage of using a panel of MoAbs with different antigenic specificities. Further study is needed to determine the prognostic significance of bone marrow involvement established by immunodetection.
利用三种针对小细胞肺癌（SCLC）产生的单克隆抗体（MoAb），通过间接免疫荧光法研究骨髓转移的检测。这些抗体分别命名为抗-LCA1、-LCA2和-LCA3，可识别SCLC细胞表面三种不同的抗原。对74例不同患者的84份骨髓样本进行了研究。通过MoAb染色在32份样本（38%）中发现了肿瘤细胞，而使用传统形态学方法仅阳性鉴定出10份样本（12%）。形态学阳性的标本中有9份与至少两种单克隆抗体呈反应性。在通过免疫荧光证实为阳性的32份样本中，观察到重要的抗原变异性。抗-LCA1识别出62%的肿瘤细胞，抗-LCA2和抗-LCA3识别出53%的肿瘤细胞。由于在34例被归类为局限性疾病的患者中，有26%通过荧光法检测到骨髓受累，因此定义了一个预后仍不确定的局限性疾病患者新亚组。我们的结果证实了免疫检测在SCLC骨髓转移诊断中的实用性，并强调了使用具有不同抗原特异性的一组MoAb的优势。需要进一步研究以确定通过免疫检测确定的骨髓受累的预后意义。
