用于体外检测人骨髓中小细胞肺癌转移灶的单克隆抗体。

Monoclonal antibodies for the in vitro detection of small cell lung cancer metastases in human bone marrow.

作者信息

Lebacq-Verheyden A M, Neirynck A, Ravoet A M, Humblet Y, Oie H, Linnoila I, Gazdar A F, Minna J D, Symann M

机构信息

Unité de Recherches sur les Maladies du Sang, Faculty of Medicine, University of Louvain, Brussels, Belgium.

出版信息

Eur J Cancer Clin Oncol. 1988 Feb;24(2):137-45. doi: 10.1016/0277-5379(88)90244-1.

DOI:10.1016/0277-5379(88)90244-1

PMID:2833399

Abstract

Three rat monoclonal antibodies were selected for the immunodetection of small cell lung cancer metastases in bone marrow and other hematologic samples. By membrane immunofluorescence, they define three distinct surface antigens here termed lung cancer-associated antigens or LCAs. The latter are widely expressed on small cell lung cancer and non-small cell lung cancer cells/cell lines, but not detectable on a variety of normal and transformed bone marrow, blood and lymphoid cells. Anti-LCA1 (IgM) is similar to the many anti-lacto-N-fucopentaose III IgM antibodies rasied against human tumors. In contrast, anti-LCA2 (IgG2b) and anti-LCA3 (IgG2a) define surface proteins of 29, 32, 41 and 98 kilodaltons, respectively, that have not been reported earlier. These three reagents have immunodiagnostic potential, since in combination they label all 49 lung cancer cell lines tested. Their ability to detect lung cancer metastases in patient's bone marrow samples is documented in an accompanying paper.

摘要

选择了三种大鼠单克隆抗体，用于免疫检测骨髓和其他血液样本中的小细胞肺癌转移灶。通过膜免疫荧光法，它们确定了三种不同的表面抗原，这里称为肺癌相关抗原或LCA。后者在小细胞肺癌和非小细胞肺癌细胞/细胞系中广泛表达，但在多种正常和转化的骨髓、血液及淋巴细胞中无法检测到。抗LCA1（IgM）与许多针对人类肿瘤产生的抗乳糖-N-岩藻五糖III IgM抗体相似。相比之下，抗LCA2（IgG2b）和抗LCA3（IgG2a）分别确定了分子量为29、32、41和98千道尔顿的表面蛋白，这些蛋白此前未见报道。这三种试剂具有免疫诊断潜力，因为它们联合使用时可标记所有检测的49种肺癌细胞系。它们在患者骨髓样本中检测肺癌转移灶的能力在随附论文中已有记载。