Pangesti Nurmi, Vandenbrande Simon, Pineda Ana, Dicke Marcel, Raaijmakers Jos M, Van Loon Joop J A
Laboratory of Entomology, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), PO Box 50, 6700 AB Wageningen, The Netherlands.
FEMS Microbiol Ecol. 2017 Apr 1;93(4). doi: 10.1093/femsec/fix038.
Plant growth-promoting microbes residing on the roots may cooperate or compete, thereby affecting their collective benefit to the host plant. Pseudomonas simiae WCS417r (formerly known as P. fluorescens WCS417r) and Pseudomonas fluorescens SS101 are well known for their ability to induce systemic resistance in Arabidopsis. Here, we evaluate how these species interact on the roots of Arabidopsis thaliana Col-0 and how their co-inoculation affects plant defense to the leaf-chewing herbivore Mamestra brassicae and plant growth promotion. WCS417r and SS101, applied individually to root tips or at two different positions along the roots, established similar population densities on Arabidopsis roots. When co-inoculated at the same position on the roots, however, WCS417r established significantly higher population densities than SS101. Both upon single inoculation and co-inoculation, the two pseudomonads induced the same level of induced systemic resistance against the caterpillar M. brassicae and the same increase in plant biomass. These results suggest that combined inoculation of both Pseudomonas strains does not significantly modify the plant's defensive capacity compared to individual inoculation, resulting in a similar effect on performance of the generalist herbivore M. brassicae.
定殖于根部的促植物生长微生物可能会相互协作或竞争，从而影响它们对寄主植物的总体益处。西梅假单胞菌WCS417r（以前称为荧光假单胞菌WCS417r）和荧光假单胞菌SS101以其在拟南芥中诱导系统抗性的能力而闻名。在此，我们评估了这些菌株在拟南芥Col-0根部的相互作用方式，以及它们共同接种如何影响植物对食叶害虫甘蓝夜蛾的防御和植物生长促进作用。将WCS417r和SS101单独施用于根尖或沿根的两个不同位置时，它们在拟南芥根上建立了相似的种群密度。然而，当在根的同一位置共同接种时，WCS417r建立的种群密度显著高于SS101。在单独接种和共同接种时，这两种假单胞菌诱导对毛虫甘蓝夜蛾的系统抗性水平相同，并且植物生物量的增加也相同。这些结果表明，与单独接种相比，两种假单胞菌菌株联合接种不会显著改变植物的防御能力，对多食性害虫甘蓝夜蛾的表现产生类似影响。
