Miller V L, Falkow S
Department of Medical Microbiology, Stanford University, California 94305.
Infect Immun. 1988 May;56(5):1242-8. doi: 10.1128/iai.56.5.1242-1248.1988.
Virulent strains of Yersinia enterocolitica cause disease syndromes ranging from mild gastroenteritis to lymphadenitis and septicemia. The ability of these bacteria to invade intestinal epithelial cells to gain access to the reticuloendothelial system is thought to be an important aspect of their virulence. We report here on the cloning of two Y. enterocolitica chromosomal loci, inv and ail, each of which confers an invasive phenotype on Escherichia coli HB101. The inv locus allows a uniformly high level of invasion in several tissue culture lines and is homologous to the inv gene of Yersinia pseudotuberculosis. The second locus, ail, shows more host specificity than inv in that it allows invasion to a variable degree of some cell lines (e.g., HEp-2, HEC1B, and CHO cells) but allows no invasion of others (e.g., Madin-Darby canine kidney cells).
肠炎耶尔森菌的强毒株可引发多种疾病综合征，从轻度肠胃炎到淋巴结炎及败血症不等。这些细菌侵入肠道上皮细胞以进入网状内皮系统的能力被认为是其毒力的一个重要方面。我们在此报告克隆了肠炎耶尔森菌的两个染色体位点，即inv和ail，它们各自赋予大肠杆菌HB101侵袭表型。inv位点在多个组织培养细胞系中均能实现一致的高水平侵袭，并且与假结核耶尔森菌的inv基因同源。第二个位点ail比inv表现出更多的宿主特异性，因为它能使某些细胞系（如HEp - 2、HEC1B和CHO细胞）在不同程度上发生侵袭，但对其他细胞系（如Madin - Darby犬肾细胞）则不引发侵袭。
