Blake Jocelin S, Trumpatori Brian J, Mathews Kyle G, Griffith Emily H
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina.
Department of Surgery, Veterinary Specialty Hospital of the Carolinas, Cary, North Carolina.
Vet Surg. 2017 May;46(4):501-506. doi: 10.1111/vsu.12648. Epub 2017 Mar 23.
To determine any loss of function of a single-use bipolar vessel sealing device after repeated sterilization and use on canine carotid arteries.
Ex vivo; randomized block design (dog).
Carotid artery segments (n = 90) from 15 canine cadavers.
Six 10-mm LigaSure Atlas 20 cm devices (Covidien Inc, Minneapolis, Minnesota) were used to seal canine carotid artery segments in one cadaver, then resterilized using ethylene oxide and reused in a second cadaver, and so on, for a total of 15 uses/15 cadavers per LigaSure. Sealing times and bursting pressures of each carotid artery segment after each use were recorded. Charring and sticking at each seal location was qualitatively assessed.
Mean (SD) sealing time for all segments was 5.3 (1.4) seconds, ranging from 2.7 to 9.5 seconds. There was no significant change in sealing time for successive uses (P = .117). The mean (SD) bursting pressure for all segments was 1041.3 (316.7) mm Hg, ranging from 355 to 1555 mm Hg. There was no change in bursting pressure across uses (P = .57). Moderate sticking and charring occurred at each time point but was not subjectively different throughout the study.
The LigaSure Atlas vessel sealing device can be used and resterilized with ethylene oxide up to 15 times with no change in sealing time or bursting pressure of the seal.
确定一次性使用的双极血管封闭装置在反复灭菌并用于犬类颈动脉后是否存在功能丧失。
体外研究；随机区组设计（犬类）。
来自15只犬类尸体的颈动脉段（n = 90）。
使用六个10毫米的LigaSure Atlas 20厘米装置（柯惠医疗公司，明尼阿波利斯，明尼苏达州）在一只尸体中封闭犬类颈动脉段，然后使用环氧乙烷进行再灭菌，并在第二只尸体中重复使用，以此类推，每个LigaSure总共使用15次/15只尸体。记录每次使用后每个颈动脉段的封闭时间和爆破压力。对每个封闭位置的炭化和粘连情况进行定性评估。
所有段的平均（标准差）封闭时间为5.3（1.4）秒，范围为2.7至9.5秒。连续使用时封闭时间无显著变化（P = 0.117）。所有段的平均（标准差）爆破压力为1041.3（316.7）毫米汞柱，范围为355至1555毫米汞柱。不同次使用间爆破压力无变化（P = 0.57）。在每个时间点均出现中度粘连和炭化，但在整个研究过程中主观上无差异。
LigaSure Atlas血管封闭装置可以使用环氧乙烷进行再灭菌，最多可使用15次，封闭时间或封闭的爆破压力均无变化。
