Tanaka K, Okamoto Y, Nagaya Y, Nishimura F, Takeoka A, Hanada S, Kohno S, Kawai M
Toxicology Laboratory, Toray Industries, Otsu, Japan.
Int Arch Allergy Appl Immunol. 1988;85(4):392-7. doi: 10.1159/000234540.
An experimental model of nasal allergy has been developed in guinea pigs by intranasal application of 2,4-toluene diisocyanate (TDI). A 10% TDI solution in ethyl acetate was painted onto the nasal vestibuli of the animals once a day for 5-10 days. During the course of repeated application of TDI, the number of animals which secreted rhinorrhea containing eosinophils increased. Morphological survey of the nasal mucosa showed infiltration of eosinophils and some other changes indicative of acute inflammation. Moreover, mast cells were found not only in the subepithelial connective tissue but also in the epithelial layer. Nasal mucus obtained from the mucosa has been found to be an effective test material for studies of nasal allergy. A striking decrease of specific granules was found in some mast cells contained in the mucus. In parallel with the symptomatology, biochemical and serological studies suggested the involvement of type I allergy in the experimental system; TDI-specific histamine release from the nasal mucosa and positive passive cutaneous anaphylaxis were found 3 weeks after the application of TDI.
通过向豚鼠鼻内施用2,4 - 甲苯二异氰酸酯（TDI）建立了鼻过敏实验模型。将10%的TDI乙酸乙酯溶液每天涂于动物鼻前庭，持续5 - 10天。在重复施用TDI的过程中，分泌含嗜酸性粒细胞鼻漏的动物数量增加。鼻黏膜的形态学检查显示嗜酸性粒细胞浸润及一些其他提示急性炎症的变化。此外，不仅在皮下结缔组织中发现了肥大细胞，在上皮层中也发现了肥大细胞。从黏膜获得的鼻黏液已被发现是研究鼻过敏的有效测试材料。在黏液中的一些肥大细胞中发现特异性颗粒显著减少。与症状学一致，生化和血清学研究表明I型过敏参与了该实验系统；在施用TDI 3周后，发现鼻黏膜有TDI特异性组胺释放以及阳性被动皮肤过敏反应。
