Lv Yan, Zhang Liang, Li Na, Mai Naiken, Zhang Yu, Pan Shuyi
Department of Hyperbaric Oxygen, PLA Navy General Hospital, Beijing 100048, P.R. China.
Can J Physiol Pharmacol. 2017 Dec;95(12):1389-1395. doi: 10.1139/cjpp-2016-0528. Epub 2017 Mar 23.
Geraniol, a plant-derived monoterpene, has been extensively studied and showed a wide variety of beneficial effects. The aim of this study was to investigate the therapeutic effect of geraniol on functional recovery and neuropathic pain in rats with spinal cord injury (SCI). Rats received a clip-compression SCI and were treated with geraniol 6 h following SCI. Treatment of SCI rats with geraniol markedly improved locomotor function, and reduced sensitivity to the mechanical allodynia and thermal hyperalgesia. Treatment of SCI rats with geraniol increased NeuN-positive cells, suppressed expression of glial fibrillary acidic protein, and reduced activity of caspase-3 in the injured region. Treatment of SCI rats with geraniol reduced levels of malondialdehyde and 3-nitrotyrosine, upregulated protein expression of nuclear factor-erythroid 2-related factor 2 and heme oxygenase 1, and suppressed expression of inducible nitric oxide synthase in the injured region. In addition, treatment of SCI rats with geraniol downregulated protein expression of N-methyl-d-aspartate receptor 1 and reduced the number of CD68-positive cells and protein levels of TNF-α in the injured region. In conclusion, geraniol significantly promoted the recovery of neuronal function and attenuated neuropathic pain after SCI.
香叶醇是一种植物源单萜，已得到广泛研究并显示出多种有益作用。本研究的目的是探讨香叶醇对脊髓损伤（SCI）大鼠功能恢复和神经性疼痛的治疗作用。大鼠接受夹压性脊髓损伤，并在脊髓损伤后6小时用香叶醇治疗。用香叶醇治疗脊髓损伤大鼠可显著改善运动功能，并降低对机械性异常性疼痛和热痛觉过敏的敏感性。用香叶醇治疗脊髓损伤大鼠可增加NeuN阳性细胞，抑制胶质纤维酸性蛋白的表达，并降低损伤区域caspase-3的活性。用香叶醇治疗脊髓损伤大鼠可降低丙二醛和3-硝基酪氨酸水平，上调核因子红细胞2相关因子2和血红素加氧酶1的蛋白表达，并抑制损伤区域诱导型一氧化氮合酶的表达。此外，用香叶醇治疗脊髓损伤大鼠可下调N-甲基-D-天冬氨酸受体1的蛋白表达，并减少损伤区域CD68阳性细胞的数量和肿瘤坏死因子-α的蛋白水平。总之，香叶醇可显著促进脊髓损伤后神经元功能的恢复并减轻神经性疼痛。
