Khoshnazar Seyedeh Mahdieh, Mohagheghi Mohammad, Rahimi Sahar, Dabiri Shahriar, Shahrokhi Nader, Shafieipour Sara
Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Jan 22. doi: 10.1007/s00210-025-03802-y.
Intestinal ischemia-reperfusion injury (IIR/I) significantly increases morbidity and mortality. This study examines the therapeutic effects of geraniol (GNL), which is noted for its anti-inflammatory and antioxidant properties, on intestinal I/R injury in rats. Forty-nine male Wistar-Albino rats were divided into seven groups. After 30 min of ischemia and subsequent reperfusion for either 1 or 6 h, intestinal and hepatic tissues were analyzed. Biochemical assessments measured malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO), and nitric oxide (NO) activities. Inflammatory and apoptotic markers were evaluated using qRT-PCR and ELISA, and apoptotic cell rates were determined by flow cytometry. GNL treatment significantly protected intestinal and hepatic tissues, enhancing antioxidant enzyme activity and normalizing MDA and NO activities. It demonstrated notable anti-inflammatory effects at 100 and 200 mg/kg doses, reducing TNF-α, IL-1β, and IL-6 levels while decreasing tissue MPO content. GNL also increased Bcl-2 levels and decreased Bax levels, indicating anti-apoptotic effects. Serum activities of ALT, AST, and creatine kinase were lower in GNL-treated rats, and flow cytometry showed a reduction in apoptotic cells. GNL effectively mitigated oxidative stress and histopathological damage from intestinal I/R injury by reducing pro-apoptotic markers and increasing anti-apoptotic markers, highlighting its protective effects.
肠缺血再灌注损伤（IIR/I）显著增加发病率和死亡率。本研究考察了以其抗炎和抗氧化特性而闻名的香叶醇（GNL）对大鼠肠缺血/再灌注损伤的治疗作用。49只雄性Wistar白化大鼠被分为7组。在缺血30分钟并随后再灌注1小时或6小时后，对肠组织和肝组织进行分析。生化评估测量丙二醛（MDA）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、髓过氧化物酶（MPO）和一氧化氮（NO）的活性。使用qRT-PCR和ELISA评估炎症和凋亡标志物，并通过流式细胞术测定凋亡细胞率。GNL治疗显著保护了肠组织和肝组织，增强了抗氧化酶活性，并使MDA和NO活性恢复正常。它在100和200mg/kg剂量下显示出显著的抗炎作用，降低了TNF-α、IL-1β和IL-6水平，同时降低了组织MPO含量。GNL还增加了Bcl-2水平并降低了Bax水平，表明具有抗凋亡作用。GNL治疗组大鼠的血清ALT、AST和肌酸激酶活性较低，流式细胞术显示凋亡细胞减少。GNL通过减少促凋亡标志物并增加抗凋亡标志物，有效减轻了肠缺血/再灌注损伤引起的氧化应激和组织病理学损伤，突出了其保护作用。
