Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Cell Syst. 2017 Mar 22;4(3):262-275. doi: 10.1016/j.cels.2017.02.010.
Rational approaches to modifying cells to make molecules of interest are of substantial economic and scientific interest. Most of these efforts aim at the production of native metabolites, expression of heterologous biosynthetic pathways, or protein expression. Reviews of these topics have largely focused on individual strategies or cell types, but collectively they fall under the broad umbrella of a growing field known as cell factory engineering. Here we condense >130 reviews and key studies in the art into a meta-review of cell factory engineering. We identified 33 generic strategies in the field, all applicable to multiple types of cells and products, and proven successful in multiple major cell types. These apply to three major categories: production of native metabolites and/or bioactives, heterologous expression of biosynthetic pathways, and protein expression. This meta-review provides general strategy guides for the broad range of applications of rational engineering of cell factories.
理性的方法来修饰细胞以制造感兴趣的分子具有重要的经济和科学意义。这些努力大多旨在生产天然代谢物、表达异源生物合成途径或蛋白质表达。这些主题的综述主要集中在个别策略或细胞类型上，但它们共同属于一个日益壮大的领域，称为细胞工厂工程。在这里，我们将 130 多篇综述和该领域的重要研究压缩成一个细胞工厂工程的元综述。我们确定了该领域的 33 种通用策略，这些策略适用于多种类型的细胞和产品，并已在多种主要细胞类型中得到成功应用。这些策略适用于三个主要类别：生产天然代谢物和/或生物活性物质、异源表达生物合成途径和蛋白质表达。这个元综述为细胞工厂的理性工程的广泛应用提供了一般策略指南。
