对大肠杆菌中所有64种密码子的翻译起始进行测量。
Measurements of translation initiation from all 64 codons in E. coli.
作者信息
Hecht Ariel, Glasgow Jeff, Jaschke Paul R, Bawazer Lukmaan A, Munson Matthew S, Cochran Jennifer R, Endy Drew, Salit Marc
机构信息
Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.
Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.
出版信息
Nucleic Acids Res. 2017 Apr 20;45(7):3615-3626. doi: 10.1093/nar/gkx070.
Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the 'start codons' for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 remaining codons. Here, we quantified translation initiation of green fluorescent protein and nanoluciferase in E. coli from all 64 triplet codons and across a range of DNA copy number. We detected initiation of protein synthesis above measurement background for 47 codons. Translation from non-canonical start codons ranged from 0.007 to 3% relative to translation from AUG. Translation from 17 non-AUG codons exceeded the highest reported rates of non-cognate codon recognition. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems.
我们对翻译的理解是构建生命系统能力的基础。在大肠杆菌中，标准起始密码子（AUG）和一些近同源密码子（GUG、UUG）被视为翻译起始的“起始密码子”。通常认为翻译不会从其余61个密码子起始。在此，我们对大肠杆菌中绿色荧光蛋白和纳米荧光素酶从所有64个三联体密码子起始的翻译起始情况进行了定量，并涵盖了一系列DNA拷贝数。我们检测到47个密码子的蛋白质合成起始高于测量背景。相对于从AUG起始的翻译，从非标准起始密码子起始的翻译范围为0.007%至3%。从17个非AUG密码子起始的翻译超过了已报道的最高非同源密码子识别率。非标准起始密码子的翻译起始可能在天然和合成生物系统中都有助于肽的合成。
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