起始密码子结构对大肠杆菌翻译的影响。

Effect of structure of the initiator codon on translation in E. coli.

作者信息

Neplyueva V S, Kalinina T I, Smirnov V D

机构信息

D.I. Ivanovsky Institute of Virology, Academy of Medical Sciences, Moscow, USSR.

出版信息

FEBS Lett. 1988 May 23;232(2):369-71. doi: 10.1016/0014-5793(88)80771-3.

DOI:10.1016/0014-5793(88)80771-3

PMID:2967775

Abstract

A set of plasmids carrying different initiator codons--either AUG, or GUG, or UUG, or CUG (as a control) in the hybrid gene lacIZ--was constructed by using synthetic oligonucleotides. GUG and UUG codons were demonstrated to be 2-3 times less effective than AUG in translation initiation. Furthermore, the correlation between the efficiencies of different initiator codons in translation initiation proved to vary, depending on the phase of bacterial growth. The rarely occurring usage in nature of the initiator codons GUG and UUG is supposed to be due to the particular role played by the initiator triplets in regulation of gene expression.

摘要

通过使用合成寡核苷酸构建了一组携带不同起始密码子（杂交基因lacIZ中的AUG、GUG、UUG或作为对照的CUG）的质粒。结果表明，GUG和UUG密码子在翻译起始中的效率比AUG低2至3倍。此外，不同起始密码子在翻译起始中的效率之间的相关性被证明会因细菌生长阶段而异。起始密码子GUG和UUG在自然界中很少出现，推测这是由于起始三联体在基因表达调控中所起的特殊作用。