Fort Chelsea, Jourdan Thomas, Curtis Byron
Office of the Chief Medical Examiner of Oklahoma, 901 N. Stonewall Avenue, Oklahoma City, OK 73117, USA.
Forensic Science Institute, University of Central Oklahoma, 100 N. University Drive, Edmond, OK 73034, USA.
J Anal Toxicol. 2017 Jun 1;41(5):360-366. doi: 10.1093/jat/bkx015.
The focus of this study was to determine the stability of four synthetic cannabinoids, XLR-11, UR-144, AB-Pinaca and AB-Fubinaca in biological specimens for the purpose of casework processing prioritization. The study used human whole blood spiked with the compounds of interest to mimic real forensic laboratory samples submitted for synthetic cannabinoid analysis. The spiked whole blood specimens were incubated under one of three temperature conditions: room or ambient (22°C), refrigerated (4°C) and frozen (-20°C) for a period of 12 weeks. Study specimens were then extracted using a forward alkaline extraction at pH 10.2 and analyzed using a liquid chromatograph tandem mass spectrometer (LC-MS-MS). Under all incubation conditions results showed that AB-Fubinaca, AB-Pinaca and UR-144 were relatively stable while XLR-11 significantly degraded at ambient and refrigerated conditions. Frozen storage conditions were the only tested parameter able to preserve and stabilize all four compounds over the three month period. Therefore, it should be suggested that forensic blood evidence suspected of containing synthetic cannabinoid compounds should be stored in frozen conditions.
本研究的重点是确定四种合成大麻素XLR-11、UR-144、AB-Pinaca和AB-Fubinaca在生物样本中的稳定性，以便对案件处理进行优先级排序。该研究使用添加了相关化合物的人体全血来模拟提交给合成大麻素分析的真实法医实验室样本。添加了化合物的全血样本在三种温度条件之一（室温或环境温度（22°C）、冷藏（4°C）和冷冻（-20°C））下孵育12周。然后使用pH值为10.2的正向碱性萃取法对研究样本进行萃取，并使用液相色谱串联质谱仪（LC-MS-MS）进行分析。在所有孵育条件下，结果表明AB-Fubinaca、AB-Pinaca和UR-144相对稳定，而XLR-11在环境温度和冷藏条件下显著降解。冷冻储存条件是在三个月期间能够保存和稳定所有四种化合物的唯一测试参数。因此，建议怀疑含有合成大麻素化合物的法医血液证据应保存在冷冻条件下。
