Lillehoj Hyun Soon, Jang Seung Ik, Panebra Alfredo, Lillehoj Erik Peter, Dupuis Laurent, Ben Arous Juliette, Lee Seung Kyoo, Oh Sung Taek
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Institute of Health and Environment, Daejeon Metropolitan City, Daejeon 305-338, Korea.
Asian-Australas J Anim Sci. 2017 Oct;30(10):1478-1485. doi: 10.5713/ajas.17.0053. Epub 2017 Mar 21.
The effects of vaccinating 18-day-old chicken embryos with the combination of recombinant profilin plus () NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using an ()/ co-infection NE disease model that we previously developed.
Eighteen-day-old broiler embryos were injected with 100 μL of phosphate-buffered saline, profilin, profilin plus necrotic enteritis B-like (NetB), profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101). After post-hatch birds were challenged with our NE experimental disease model, body weights, intestinal lesions, serum antibody levels to NetB, and proinflammatory cytokine and chemokine mRNA levels in intestinal intraepithelial lymphocytes were measured.
Chickens vaccinated with recombinant profilin plus NetB proteins/IMS106 and recombinant profilin plus NetB proteins/IMS101 showed significantly increased body weight gains and reduced gut damages compared with the profilin-only group, respectively. Greater antibody response to NetB toxin were observed in the profilin plus NetB/IMS 106, and profilin plus NetB/IMS 101 groups compared with the other three vaccine/adjuvant groups. Finally, diminished levels of transcripts encoding for proinflammatory cytokines such as lipopolysaccharide-induced tumor necrosis factor-α factor, tumor necrosis factor superfamily 15, and interleukin-8 were observed in the intestinal lymphocytes of chickens injected with profilin plus NetB toxin in combination with IMS 106, and profilin plus NetB toxin in combination with IMS 101 compared with profilin protein alone bird.
These results suggest that the Montanide IMS adjuvants potentiate host immunity to experimentally-induced avian NE when administered in conjunction with the profilin and NetB proteins, and may reduce disease pathology by attenuating the expression of proinflammatory cytokines and chemokines implicated in disease pathogenesis.
利用我们之前建立的产气荚膜梭菌/球虫共感染坏死性肠炎（NE）疾病模型，研究在18日龄鸡胚中接种重组肌动蛋白结合蛋白与NetB蛋白混合物（混合于Montanide IMS佐剂中）对鸡针对坏死性肠炎的免疫反应的影响。
给18日龄的肉鸡胚注射100μL磷酸盐缓冲盐水、肌动蛋白结合蛋白、肌动蛋白结合蛋白加坏死性肠炎B样毒素（NetB）、肌动蛋白结合蛋白加NetB/Montanide佐剂（IMS 106）以及肌动蛋白结合蛋白加Net - B/Montanide佐剂（IMS 101）。在雏鸡用我们的NE实验疾病模型进行攻毒后，测量其体重、肠道病变、针对NetB的血清抗体水平以及肠道上皮内淋巴细胞中促炎细胞因子和趋化因子的mRNA水平。
与仅接种肌动蛋白结合蛋白的组相比，接种重组肌动蛋白结合蛋白加NetB蛋白/IMS106和重组肌动蛋白结合蛋白加NetB蛋白/IMS101的鸡体重增加显著，肠道损伤减轻。与其他三个疫苗/佐剂组相比，肌动蛋白结合蛋白加NetB/IMS 106组和肌动蛋白结合蛋白加NetB/IMS 101组对NetB毒素的抗体反应更强。最后,与仅接种肌动蛋白结合蛋白相比，接种肌动蛋白结合蛋白加NetB毒素与IMS 106组合以及肌动蛋白结合蛋白加NetB毒素与IMS 101组合的鸡的肠道淋巴细胞中，脂多糖诱导的肿瘤坏死因子-α、肿瘤坏死因子超家族15和白细胞介素-8等促炎细胞因子的转录水平降低。
这些结果表明，Montanide IMS佐剂与肌动蛋白结合蛋白和NetB蛋白联合使用时，可增强宿主对实验性诱导的禽坏死性肠炎的免疫力，并可能通过减弱与疾病发病机制相关的促炎细胞因子和趋化因子的表达来减轻疾病病理变化。
