Jeong Haeyoung, Lee Seung-Won, Kim Sun Hong, Kim Eun-Youn, Kim Sinyeon, Yoon Sung Ho
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
SeqGenesis, Daejeon 34111, Republic of Korea.
J Microbiol Biotechnol. 2017 Jun 28;27(6):1171-1179. doi: 10.4014/jmb.1702.02021.
Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily because of its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-type and its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitive was exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed. Intriguingly, the evolved butanol-tolerant strain behaved similarly in both the absence and presence of butanol. Among the mutations occurring in the evolved strain, cis-regulatory mutations may be the cause of butanol tolerance. This study provides a foundation for the rational design of the metabolic and regulatory pathways for enhanced biofuel production.
丁醇是乙醇的一种有前景的替代品，可用于运输燃料以及汽油和柴油燃料的添加剂。微生物生产丁醇具有挑战性，主要是因为其毒性和低产量。在此，我们比较了野生型及其耐丁醇进化菌株的转录组和表型组，以了解负责丁醇耐受性的整体细胞生理学和代谢。当祖先的丁醇敏感菌株暴露于丁醇时，参与呼吸机制和氧化应激的基因活性受到高度干扰。有趣的是，进化后的耐丁醇菌株在有无丁醇的情况下表现相似。在进化菌株中发生的突变中，顺式调节突变可能是丁醇耐受性的原因。这项研究为合理设计用于提高生物燃料产量的代谢和调节途径奠定了基础。
