Houser C R, Olsen R W, Richards J G, Möhler H
Neurology Service, Veterans Administration Medical Center, West Los Angeles, Wadsworth Division, California.
J Neurosci. 1988 Apr;8(4):1370-83. doi: 10.1523/JNEUROSCI.08-04-01370.1988.
Monoclonal antibodies to a purified benzodiazepine/GABAA receptor complex from bovine cerebral cortex were used to determine the localization of this receptor in immunohistochemical preparations of the human hippocampal formation. The regional localization of the receptor was compared with the autoradiographic distribution of binding sites for a benzodiazepine antagonist, Ro 15-1788, and very similar patterns were observed. The highest levels of labeling were present in the molecular layer of the dentate gyrus, where much of the immunoreaction product was localized on the dendrites of granule cells. Lower levels of staining were observed in the granule cell layer, where reaction product was distributed around the perimeter of granule cell somata. The staining patterns varied among the different fields of the hippocampus. Labeling was lowest in the CA3 field, increased somewhat in the CA2 field, and was highest in the CA1 field. Within CA1, a laminar pattern was observed. Moderate to high levels of staining were present in strata oriens, pyramidale, radiatum, and moleculare, whereas less staining was observed in stratum lacunosum. The cellular localization of the receptor also differed among the hippocampal fields. Very little reaction product was observed on pyramidal neurons in the CA3 field, but labeling of pyramidal neurons appeared to increase progressively throughout CA2, CA1, and the subiculum. Nonpyramidal neurons were prominently labeled in all hippocampal fields. These results indicate that the benzodiazepine/GABAA receptor is heterogeneously distributed among the different fields and on different neuronal cell types of the human hippocampus.
利用针对从牛大脑皮层纯化的苯二氮䓬/GABAA受体复合物的单克隆抗体，在人类海马结构的免疫组织化学制剂中确定该受体的定位。将该受体的区域定位与苯二氮䓬拮抗剂Ro 15 - 1788结合位点的放射自显影分布进行比较，观察到非常相似的模式。标记水平最高的区域位于齿状回分子层，其中大部分免疫反应产物定位于颗粒细胞的树突上。在颗粒细胞层观察到较低水平的染色，反应产物分布在颗粒细胞胞体的周边。海马不同区域的染色模式各不相同。CA3区的标记最低，CA2区有所增加，CA1区最高。在CA1区内，观察到一种分层模式。在海马层、锥体层、辐射层和分子层中存在中度到高度的染色，而在腔隙层中观察到的染色较少。受体在细胞上的定位在海马各区域之间也有所不同。在CA3区的锥体神经元上观察到极少的反应产物，但在整个CA2、CA1和下托中，锥体神经元的标记似乎逐渐增加。在所有海马区域中，非锥体神经元都有明显的标记。这些结果表明，苯二氮䓬/GABAA受体在人类海马的不同区域和不同神经元细胞类型中呈异质性分布。
