Pitkänen A, Amaral D G
University of Kuopio, Department of Neurology, Finland.
J Comp Neurol. 1993 May 1;331(1):37-74. doi: 10.1002/cne.903310104.
The distribution of parvalbumin-immunoreactive cells and fibers in the various fields of the hippocampal formation was studied in the macaque monkey. Parvalbumin-immunoreactive neurons had aspiny or sparsely spiny dendrites that often had a beaded appearance; most resembled classically identified interneurons. Parvalbumin-immunoreactive fibers and terminals were confined to certain laminae in each field and generally had a pericellular distribution. In the dentate gyrus, there was a dense pericellular plexus of immunoreactive terminals in the granule cell layer. Except for a narrow supragranular zone, there was a marked paucity of terminals in the molecular and polymorphic cell layers. Immunoreactive neurons were mainly located immediately subjacent to the granule cell layer and comprised a variety of morphological cell types. The three fields of the hippocampus proper (CA3, CA2, and CA1) demonstrated differences in their parvalbumin staining characteristics. In CA3, there was a prominent pericellular terminal plexus in the pyramidal cell layer that was densest distally (closer to CA2). Immunoreactive cells were located either in the pyramidal cell layer, where many had a pyramidal shape and prominent apical and basal dendrites, or in stratum oriens. CA2 had a staining pattern similar to that in CA3, though both the number of labeled cells and the density of the pericellular terminal plexus were greater in CA2. In CA1, there was a markedly lower number of parvalbumin-labeled cells than in CA3 and CA2 and the cells tended to be located in the deep part of the pyramidal cell layer or in stratum oriens. The pyramidal cell layer of CA1 contained a pericellular terminal plexus that was substantially less dense than in CA3 and CA2. At the border between CA1 and the subiculum there was a marked increase in the number of parvalbumin-immunoreactive neurons. The positive cells were scattered throughout the pyramidal cell layer of the subiculum and comprised a variety of sizes and shapes. Terminal labeling was higher in the pyramidal cell layer of the subiculum than in CA1. Layer II of the presubiculum had one of the highest densities of fiber and terminal labeling in the hippocampal formation. The density of staining was lower in the superficial portion of the layer where linear cartridges of presumed axo-axonic synapses were common. A large number of parvalbumin-immunoreactive cells were scattered throughout layer II of the presubiculum; small, spherical, multipolar cells were commonly observed in layer I. The parasubiculum had a somewhat lower density of positive cells and fibers than the presubiculum.(ABSTRACT TRUNCATED AT 400 WORDS)
在猕猴中研究了海马结构各区域中小清蛋白免疫反应性细胞和纤维的分布。小清蛋白免疫反应性神经元具有无棘或棘少的树突，这些树突常呈串珠状；大多数类似于经典鉴定的中间神经元。小清蛋白免疫反应性纤维和终末局限于每个区域的特定层，并且通常呈细胞周分布。在齿状回，颗粒细胞层有密集的免疫反应性终末细胞周丛。除了狭窄的颗粒上层区域外，分子层和多形细胞层中的终末明显稀少。免疫反应性神经元主要位于颗粒细胞层紧邻的下方，包括多种形态的细胞类型。海马体本身的三个区域（CA3、CA2和CA1）在小清蛋白染色特征上表现出差异。在CA3，锥体细胞层有一个突出的细胞周终末丛，在远端（靠近CA2）最密集。免疫反应性细胞位于锥体细胞层，其中许多细胞呈锥形，具有明显的顶树突和基底树突，或者位于海马下托。CA2的染色模式与CA3相似，尽管CA2中标记细胞的数量和细胞周终末丛的密度都更高。在CA1，小清蛋白标记细胞的数量明显低于CA3和CA2，并且细胞倾向于位于锥体细胞层的深部或海马下托。CA1的锥体细胞层含有一个细胞周终末丛，其密度明显低于CA3和CA2。在CA1和海马下托之间的边界处，小清蛋白免疫反应性神经元的数量显著增加。阳性细胞散布在海马下托的锥体细胞层中，包括各种大小和形状。海马下托锥体细胞层的终末标记高于CA1。前海马下托的II层在海马结构中具有纤维和终末标记的最高密度之一。在该层的浅部，假定的轴-轴突触线性小体常见，染色密度较低。大量小清蛋白免疫反应性细胞散布在前海马下托的II层中；在I层中常见小的、球形的、多极细胞。副海马下托的阳性细胞和纤维密度略低于前海马下托。（摘要截短于400字）
