Faron-Górecka A, Kuśmider M, Szafran-Pilch K, Kolasa M, Żurawek D, Gruca P, Papp M, Solich J, Pabian P, Dziedzicka-Wasylewska M
Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smętna Street 12, Kraków 31-343, Poland.
Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smętna Street 12, Kraków 31-343, Poland.
Neurosci Lett. 2017 Apr 24;647:147-152. doi: 10.1016/j.neulet.2017.03.034. Epub 2017 Mar 20.
Prolactin (PRL) has been shown to be altered by psychotropic drugs, including antidepressant drugs (ADs). Many studies have focused on the response to antidepressant treatment (especially related to the serotonergic system) using the fenfluramine test (PRF), however some data suggest lack of correlation between PRF and prediction of clinical response to ADs. In our study we have investigated the hypothesis that basal plasma level of prolactin is a better predictor of antidepressant treatment. We have used Chronic Mild Stress (CMS) - the animal model of depression. Rats are exposed to CMS in combination with imipramine (IMI) treatment for 5 consecutive weeks. Blood samples were collected from the rat tail vein three times: before the CMS procedure, after 2 weeks of stress and after the complete CMS procedure (after 5 weeks of stress and IMI treatment). The PRL level in plasma was determined using the commercially available ELISA kit. In CMS, anhedonia in rats is manifested by reduced consumption of sucrose solution while administration of antidepressant drugs reverses anhedonia. Some animals (ca.30%) did not respond to antidepressant therapy and were considered treatment-resistant. There was no correlation between basal PRL levels and stress response, however, from the results obtained by Spearman Rank Correlation analysis we have observed a significant negative correlation between basal PRL levels before the CMS procedure and behavioral response to IMI administration. The obtained results indicate that the basal PRL level in rat plasma correlates with a good response to treatment in the animal model of depression.
催乳素（PRL）已被证明会受到包括抗抑郁药（ADs）在内的精神药物的影响。许多研究使用芬氟拉明试验（PRF）来关注对抗抑郁治疗的反应（特别是与血清素能系统相关的反应），然而一些数据表明PRF与抗抑郁药临床反应的预测之间缺乏相关性。在我们的研究中，我们调查了催乳素基础血浆水平是抗抑郁治疗更好预测指标的假设。我们使用了慢性轻度应激（CMS）——抑郁症的动物模型。大鼠连续5周接受CMS联合丙咪嗪（IMI）治疗。从大鼠尾静脉采集三次血样：在CMS程序之前、应激2周后以及完整的CMS程序之后（应激5周和IMI治疗后）。使用市售的ELISA试剂盒测定血浆中的PRL水平。在CMS中，大鼠的快感缺失表现为蔗糖溶液消耗量减少，而给予抗抑郁药可逆转快感缺失。一些动物（约30%）对抗抑郁治疗无反应，被认为是治疗抵抗性的。基础PRL水平与应激反应之间没有相关性，然而，通过Spearman等级相关分析获得的结果显示，在CMS程序之前的基础PRL水平与IMI给药后的行为反应之间存在显著的负相关。所得结果表明，大鼠血浆中的基础PRL水平与抑郁症动物模型中的良好治疗反应相关。
