Yan Shuo, You Zi-Li, Zhao Qiu-Ying, Peng Cheng, He Gang, Gou Xiao-Jun, Lin Bin
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China; Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Commission, Chengdu University, Chengdu, China.
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Kaohsiung J Med Sci. 2015 Dec;31(12):605-12. doi: 10.1016/j.kjms.2015.10.009. Epub 2015 Nov 27.
Natural products have been widely reported as effective therapeutic alternatives for treatment of depression. Sanyuansan is a compound recipe composed of ginseng total saponins, fish oil, and valeriana. The aims of this study were to validate whether Sanyuansan has antidepressant-like effects through acute behavioral tests including the forced swimming test (FST), tail suspension test (TST), locomotor activity test, and chronic mild stress (CMS) mice model of depression. C57BL/6 mice were given oral administration of 30 mg/kg imipramine, Sanyuansan, and saline, respectively. The acute behavioral tests including the TST, FST, and locomotor activity test were done after the administration of drugs for consecutively three times (24 hours, 1 hour, and 0.5 hour prior to the tests). Furthermore, the sucrose preference and the serum corticosterone level of mice in the CMS model were examined. Sanyuansan only at 900 mg/kg markedly reduced immobility time in the TST compared with the saline-treated group of mice. Sanyuansan at doses of 225 mg/kg, 450 mg/kg, and 900 mg/kg significantly reduced immobility time of mice in the FST. Sanyuansan reversed the CMS-induced anhedonia and hyperactivation of the hypothalamus-pituitary-adrenal axis. In addition, our results showed that neither imipramine nor Sanyuansan at any dosage increased spontaneous motor activity. These results suggested that Sanyuansan induced significant antidepressant-like effects in mice in both acute and chronic animal models, which seemed unlikely to be attributed to an increase in locomotor activities of mice, and had no sedative-like effects.
天然产物作为治疗抑郁症的有效替代疗法已被广泛报道。三元散是一种由人参总皂苷、鱼油和缬草组成的复方制剂。本研究的目的是通过强迫游泳试验（FST）、悬尾试验（TST）、自发活动试验和慢性轻度应激（CMS）抑郁小鼠模型等急性行为学试验，验证三元散是否具有抗抑郁样作用。将C57BL/6小鼠分别口服给予30mg/kg丙咪嗪、三元散和生理盐水。在连续给药3次（试验前24小时、1小时和0.5小时）后进行包括TST、FST和自发活动试验在内的急性行为学试验。此外，检测CMS模型小鼠的蔗糖偏好和血清皮质酮水平。与生理盐水处理组小鼠相比，仅900mg/kg的三元散显著缩短了TST中的不动时间。225mg/kg、450mg/kg和900mg/kg剂量的三元散显著缩短了FST中小鼠的不动时间。三元散逆转了CMS诱导的快感缺失和下丘脑-垂体-肾上腺轴的过度激活。此外，我们的结果表明，任何剂量的丙咪嗪和三元散均未增加自发运动活性。这些结果表明，三元散在急性和慢性动物模型中均对小鼠产生了显著的抗抑郁样作用，这似乎不太可能归因于小鼠运动活性的增加，且无镇静样作用。
