Bellis M, Jubier-Maurin V, Dod B, Vanlerberghe F, Laurent A M, Senglat C, Bonhomme F, Roizès G
Centre National de la Recherche Scientifique, Institut de Biologie, Montpellier, France.
Mol Biol Evol. 1987 Jul;4(4):351-63. doi: 10.1093/oxfordjournals.molbev.a040449.
The presence of the L1 sequences, L1Md4 next to the pseudogene beta h3 and I12 found in the twelfth intron of the albumin gene, in certain strains of laboratory mice but not of others has led to the suggestion that these sequences were recent insertions into the Mus mus domesticus genome. To be sure that they are really recent insertions and not relics of an ancestral chromosome, we investigated the presence or absence of these sequences in populations of wild mice belonging to the semispecies M. m. domesticus and M. m. musculus as well as in other species of the genus Mus and in related murids. The sequence I12 in the albumin gene was found in 34% of the chromosomes of the wild mice belonging to M. m. domesticus and to a lesser extent (6%) in M. m. musculus. Of 114 M. m. domesticus chromosomes, L1Md4 was found in only nine, seven of which came from the same locality. Its presence was associated with the haplotype Hbbp, which is relatively rare in European populations of M. musculus. Since there was no evidence for the presence of these two L1 sequences in more distantly related species, we conclude that they are recent insertions in the M. musculus genome.
在某些品系的实验小鼠而非其他品系中，在假基因βh3旁边存在L1序列L1Md4，且在白蛋白基因的第十二个内含子中发现了I12，这使得有人提出这些序列是近期插入小家鼠基因组的。为了确定它们确实是近期插入的，而非祖先染色体的遗迹，我们调查了属于半物种小家鼠和小鼠的野生小鼠种群以及小家鼠属的其他物种和相关鼠科动物中这些序列的有无。在属于小家鼠的野生小鼠的34%的染色体中发现了白蛋白基因中的I12序列，在小鼠中发现的比例较低（6%）。在114条小家鼠染色体中，仅在9条中发现了L1Md4，其中7条来自同一地点。它的存在与单倍型Hbbp相关，Hbbp在欧洲小鼠种群中相对罕见。由于在关系更远的物种中没有证据表明存在这两个L1序列，我们得出结论，它们是小鼠基因组中的近期插入序列。
