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应激相关蛋白（SAP）基因对李属水潴留和细胞生长的双重调控。

Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus.

机构信息

Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113, Moncada, Valencia, Spain.

Department of Plant Production, Instituto de Biotecnología Vegetal-Universidad Politécnita de Cartagena (IBV-UPCT), 30202, Cartagena, Murcia, Spain.

出版信息

Sci Rep. 2017 Mar 23;7(1):332. doi: 10.1038/s41598-017-00471-7.

DOI:10.1038/s41598-017-00471-7
PMID:28336950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5428470/
Abstract

We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF)1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.

摘要

我们鉴定了一个桃基因（PpSAP1），其编码一个应激相关蛋白（SAP），包含锌指结构域 A20 和 AN1。SAP 被描述为植物物种非生物胁迫响应的调节因子，成为提高植物胁迫耐受性的潜在候选物。PpSAP1 在叶片和休眠芽中高表达，在休眠芽释放之前下调。PpSAP1 在芽中适度地被水分胁迫和热诱导表达。此外，发现 PpSAP1 在酵母双杂交系统中与多泛素蛋白强烈相互作用。在转基因李树中的过表达导致叶片形状改变，并在干旱胁迫下增加水分保持。此外，我们确定了叶片形态的改变伴随着细胞大小的减小和参与细胞生长的基因（如生长调节因子（GRF）1 样、液泡内在蛋白（TIP）样和雷帕霉素靶蛋白（TOR）样）的下调。特别是，在转基因李和桃芽中 PpSAP1 和 TOR 样的反式表达模式表明 PpSAP1 通过调节 TOR 途径在细胞扩张中发挥作用。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b50/5428470/f797db84877b/41598_2017_471_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b50/5428470/dc8b9bebed53/41598_2017_471_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b50/5428470/db284edb95fb/41598_2017_471_Fig9_HTML.jpg
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