Krejčí Jana, Legartová Soňa, Bártová Eva
Institute of Biophysics of the Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.
Stem Cells Int. 2017;2017:1021240. doi: 10.1155/2017/1021240. Epub 2017 Feb 27.
Cajal bodies (CBs) are important compartments containing accumulated proteins that preferentially regulate RNA-related nuclear events, including splicing. Here, we studied the nuclear distribution pattern of CBs in neurogenesis. In adult brains, coilin was present at a high density, but CB formation was absent in the nuclei of the choroid plexus of the lateral ventricles. Cells of the adult hippocampus were characterized by a crescent-like morphology of coilin protein. We additionally observed a 70 kDa splice variant of coilin in adult mouse brains, which was different to embryonic brains and mouse pluripotent embryonic stem cells (mESCs), characterized by the 80 kDa standard variant of coilin. Here, we also showed that depletion of coilin is induced during neural differentiation and HDAC1 deficiency in mESCs caused coilin accumulation inside the fibrillarin-positive region of the nucleoli. A similar distribution pattern was observed in adult brain hippocampi, characterized by lower levels of both coilin and HDAC1. In summary, we observed that neural differentiation and HDAC1 deficiency lead to coilin depletion and coilin accumulation in body-like structures inside the nucleoli.
卡哈尔体（CBs）是重要的区室，含有积累的蛋白质，这些蛋白质优先调节与RNA相关的核事件，包括剪接。在此，我们研究了神经发生过程中CBs的核分布模式。在成体大脑中，卷曲螺旋蛋白（coilin）高密度存在，但侧脑室脉络丛的细胞核中不存在CB形成。成体海马体的细胞以coilin蛋白呈新月样形态为特征。我们还在成年小鼠大脑中观察到一种70 kDa的coilin剪接变体，它与胚胎大脑和小鼠多能胚胎干细胞（mESCs）不同，后者以80 kDa的标准coilin变体为特征。在此，我们还表明，在神经分化过程中会诱导coilin的缺失，并且mESCs中组蛋白去乙酰化酶1（HDAC1）缺乏会导致coilin在核仁中纤维蛋白原阳性区域内积累。在成体大脑海马体中观察到类似的分布模式，其特征是coilin和HDAC1水平均较低。总之，我们观察到神经分化和HDAC1缺乏会导致coilin缺失以及coilin在核仁内类似小体的结构中积累。
