MiR-217 promotes cutaneous squamous cell carcinoma progression by targeting PTRF.

Increasing evidences have suggested that microRNAs (miRNAs) act a critical role in tumor initiation, progression and metastasis. Deregulated expression of miR-217 has been identified in various tumors. However, the expression and role of miR-217 in the development of cutaneous squamous cell carcinoma (cSCC) remain unclear. In our study, we showed that miR-217 expression was upregulated in the cSCC tissues compared to adjacent non-tumor samples. We also demonstrated that miR-217 expression was upregualted in the cSCCcSCC cell lines. Overexpression of miR-217 promoted cSCCcSCC cell growth, cell cycle and invasion. We identified Polymerase I and Transcript Release Factor (PTRF) as a direct target gene of miR-217 in the SCC13 cell. In addition, PTRF expression was downregulated in the cSCCcSCC tissues. Moreover, we demonstrated that there was a significant inverse correlation between miR-217 and PTRF expression in the cSCCcSCC. Furthermore, overexpression of PTRF could rescue miR-217's oncogenic effect on cSCC. Therefore, these results suggested that upregulation of miR-217 could contribute to development of cSCCcSCC through targeting PTRF.