Department of Dermatology, the Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui, 230601, China.
Department of Cardiology, The Second People's Hospital of Hefei, Hefei, Anhui, 230011, China.
Pathol Res Pract. 2019 Oct;215(10):152600. doi: 10.1016/j.prp.2019.152600. Epub 2019 Aug 16.
Cutaneous squamous cell carcinoma (CSCC), a class of skin tumor derived from epidermal keratinocyte, is reputed as one of the most malignant tumors globally. MicroRNAs (miRNAs) are increasingly identified as essential players in CSCC. Current study aimed to uncover the impact and mechanism of miR-1193 in CSCC. We identified the low expression of miR-1193 in CSCC cell lines. Gain- and loss-of-function assays showed that miR-1193 acted as an inhibitor of proliferation and migration in CSCC cells. Furthermore, we illustrated that miR-1193 targeted and inhibited SRY-box 4 (SOX4), and that long intergenic non-protein coding RNA 963 (LINC00963) sponged miR-1193 to upregulate SOX4 expression. Rescue assays showed that LINC00963 regulated CSCC progression through miR-1193/SOX4 axis. In conclusion, our study firstly revealed the LINC00963/miR-1193/SOX4 axis in CSCC, indicating miR-1193 as a promising biological target in CSCC progression.
皮肤鳞状细胞癌（CSCC）是一种源于表皮角质形成细胞的皮肤肿瘤，被认为是全球最恶性的肿瘤之一。microRNAs（miRNAs）越来越被认为是 CSCC 中的重要调控因子。本研究旨在揭示 miR-1193 在 CSCC 中的作用及机制。我们发现 miR-1193 在 CSCC 细胞系中表达水平较低。功能获得和缺失实验表明，miR-1193 可抑制 CSCC 细胞的增殖和迁移。此外，我们表明 miR-1193 靶向并抑制了性别决定区 Y 框 4（SOX4），而长链非编码 RNA 963（LINC00963）可通过海绵吸附 miR-1193 来上调 SOX4 的表达。挽救实验表明，LINC00963 通过 miR-1193/SOX4 轴调控 CSCC 的进展。综上所述，本研究首次揭示了 CSCC 中的 LINC00963/miR-1193/SOX4 轴，表明 miR-1193 可能是 CSCC 进展的一个有前途的生物靶点。
