Zhao Yan, Meng Qingfeng, Gao Xu, Zhang Lihua, An Lixin
Department of Allergy, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, China.
Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, China.
Am J Transl Res. 2017 Feb 15;9(2):755-761. eCollection 2017.
Mediator 19 (Med19) is a component of the mediator complex which is a co-activator for DNA-binding factors that activate transcription via RNA polymerase II. Accumulating evidence has shown that Med19 plays important roles in cancer cell proliferation and tumorigenesis. The physiological mechanism by which Med19 exerts its promoting effects in laryngocarcinoma is not yet fully understood. Here, we found that the expression of Med19 was increased in laryngocarcinoma samples from patients compared to normal bone tissues. Med19 knockdown significantly induced growth inhibition and suppressed migration in the HEp2 cell lines. Med19 knockdown also induced apoptosis in HEp2 cells via activation of caspase-3, 9 and Apaf-1. In addition, The tumorigenicity of Med19 short hairpin RNA (shRNA)-expressing cells were decreased after inoculating into nude mice. Taken together, our data suggest that Med19 acts as an oncogene in laryngocarcinoma via a possible caspase modulation pathway.
中介体19（Med19）是中介体复合物的一个组成部分，该复合物是一种DNA结合因子的共激活因子，可通过RNA聚合酶II激活转录。越来越多的证据表明，Med19在癌细胞增殖和肿瘤发生中起重要作用。Med19在喉癌中发挥促进作用的生理机制尚未完全了解。在此，我们发现与正常骨组织相比，Med19在喉癌患者样本中的表达增加。Med19敲低显著诱导HEp2细胞系生长抑制并抑制迁移。Med19敲低还通过激活caspase-3、9和Apaf-1诱导HEp2细胞凋亡。此外，将表达Med19短发夹RNA（shRNA）的细胞接种到裸鼠中后，其致瘤性降低。综上所述，我们的数据表明，Med19可能通过半胱天冬酶调节途径在喉癌中作为癌基因发挥作用。
