Pereira Nelson A M, Laranjo Mafalda, Casalta-Lopes João, Serra Arménio C, Piñeiro Marta, Pina João, Seixas de Melo J Sérgio, Senge Mathias O, Botelho M Filomena, Martelo Liliana, Burrows Hugh D, Pinho E Melo Teresa M V D
CQC, Department of Chemistry, University of Coimbra , 3004-535 Coimbra, Portugal.
Biophysics Unit, Faculty of Medicine of University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal; CIMAGO-Center of Investigation in Environment, Genetics and Oncobiology, Faculty of Medicine of University of Coimbra, Azinhaga de Santa Comba, Celas, 3004-548 Coimbra, Portugal; CNC.IBILI Consortium, IBILI, University of Coimbra, Azinhaga de Santa Comba, Celas, 3004-548 Coimbra, Portugal.
ACS Med Chem Lett. 2017 Feb 22;8(3):310-315. doi: 10.1021/acsmedchemlett.6b00476. eCollection 2017 Mar 9.
Novel near-infrared luminescent compounds based on platinum(II) 4,5,6,7-tetrahydropyrazolo[1,5-]pyridine-fused chlorins are described. These compounds have high photostability and display light emission, in particular simultaneous fluorescence and phosphorescence emission in solution at room temperature, in the biologically relevant 700-850 nm red and near-infrared (NIR) spectral region, making them excellent materials for biological imaging. The simultaneous presence of fluorescence and phosphorescence emission at room temperature, with the phosphorescence strongly quenched by oxygen whereas fluorescence remains unaffected, allows these compounds to be used as ratiometric oxygen sensors in chemical and biological media. Both steady-state (fluorescence vs phosphorescence intensities) and dynamic (dependence of phosphorescence lifetimes upon oxygen concentration) luminescence approaches can be used. Photocytotoxicity studies against human melanocytic melanoma cells (A375) indicate that these compounds display potential as photosensitizers in photodynamic therapy.
描述了基于铂（II）4,5,6,7 - 四氢吡唑并[1,5 - ]吡啶稠合二氢卟吩的新型近红外发光化合物。这些化合物具有高光稳定性，并在生物相关的700 - 850 nm红色和近红外（NIR）光谱区域显示发光，特别是在室温下在溶液中同时发出荧光和磷光，使其成为生物成像的优异材料。室温下同时存在荧光和磷光发射，其中磷光被氧气强烈猝灭而荧光不受影响，使得这些化合物能够在化学和生物介质中用作比率氧传感器。稳态（荧光与磷光强度）和动态（磷光寿命对氧浓度的依赖性）发光方法均可使用。针对人黑素细胞黑色素瘤细胞（A375）的光细胞毒性研究表明，这些化合物在光动力疗法中显示出作为光敏剂的潜力。
