一种用于在探针上检测糖蛋白和糖肽的硼酸功能化板的快速制备方法。
A Rapid Approach for Fabricating Boronic Acid-Functionalized Plates for On-Probe Detection of Glycoprotein and Glycopeptide.
作者信息
Liu Yu-Ching, Chen Chao-Jung
机构信息
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital.
Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital; Graduate Institute of Integrated Medicine, China Medical University.
出版信息
Mass Spectrom (Tokyo). 2017;6(Spec Iss):S0063. doi: 10.5702/massspectrometry.S0063. Epub 2017 Feb 24.
Abstract
We developed a rapid and simple approach without using complex mechanical or chemical protocols to fabricate boronic acid-functionalized plates for glycoprotein or glycopeptide enrichment and mass spectrometry (MS) analysis. By coating the boronic acid-functionalized silica particles on a polydimethylsiloxane (PDMS)-coated matrix-assisted laser desorption/ionization (MALDI) plate, these particles can form a firmly monolayer of particles on PDMS membrane for sample handling without peeling off. The boronic acid particles-coated PDMS plate (BP plate) was successfully applied to the enrichment of horseradish peroxidase (HRP) protein and their digested glycopeptides.
摘要
我们开发了一种快速且简单的方法，无需使用复杂的机械或化学方案来制备用于糖蛋白或糖肽富集及质谱（MS）分析的硼酸功能化板。通过将硼酸功能化的二氧化硅颗粒涂覆在聚二甲基硅氧烷（PDMS）涂层的基质辅助激光解吸/电离（MALDI）板上，这些颗粒可以在PDMS膜上形成牢固的单层颗粒，用于样品处理而不会脱落。硼酸颗粒涂覆的PDMS板（BP板）已成功应用于辣根过氧化物酶（HRP）蛋白及其消化后的糖肽的富集。
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