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2
A novel titanium dioxide-polydimethylsiloxane plate for phosphopeptide enrichment and mass spectrometry analysis.一种新型钛二氧化硅-聚二甲基硅氧烷板用于磷酸肽的富集和质谱分析。
Anal Chim Acta. 2014 Feb 17;812:105-13. doi: 10.1016/j.aca.2014.01.010. Epub 2014 Jan 15.
3
Highly efficient enrichment method for glycopeptide analyses: using specific and nonspecific nanoparticles synergistically.高效的糖肽分析富集方法：特异性和非特异性纳米颗粒协同作用。
Anal Chem. 2014 Feb 18;86(4):2057-64. doi: 10.1021/ac403236q. Epub 2014 Feb 5.
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Interaction modes and approaches to glycopeptide and glycoprotein enrichment.糖肽和糖蛋白的富集的相互作用模式和方法。
Analyst. 2014 Feb 21;139(4):688-704. doi: 10.1039/c3an01813j.
5
Simple fabrication of hydrophobic surface target for increased sensitivity and homogeneity in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of peptides, phosphopeptides, carbohydrates and proteins.简单制备疏水性表面靶标，提高肽、磷酸肽、碳水化合物和蛋白质的基质辅助激光解吸/电离飞行时间质谱分析的灵敏度和均一性。
Anal Chim Acta. 2013 Jun 14;783:31-8. doi: 10.1016/j.aca.2013.04.029. Epub 2013 Apr 25.
6
An accessible protocol for solid-phase extraction of N-linked glycopeptides through reductive amination by amine-functionalized magnetic nanoparticles.通过胺功能化磁性纳米粒子的还原胺化作用实现 N-连接糖肽的固相萃取的易操作方案。
Anal Chem. 2013 Jun 4;85(11):5535-41. doi: 10.1021/ac400733y. Epub 2013 May 23.
7
Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides.用于聚糖和糖肽的微尺度纯化和富集的棉亲水性相互作用 SPE 微吸头。
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8
Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints.在体 N-糖蛋白质组的精确定位揭示了严格的拓扑和序列限制。
Cell. 2010 May 28;141(5):897-907. doi: 10.1016/j.cell.2010.04.012.
9
On-plate enrichment of glycopeptides by using boronic acid functionalized gold-coated Si wafer.通过使用硼酸功能化的金涂覆硅片在板上对糖肽进行富集。
Proteomics. 2010 Mar;10(5):1079-86. doi: 10.1002/pmic.200900097.
10
Facile synthesis of 4-mercaptophenylboronic acid functionalized gold nanoparticles for selective enrichment of glycopeptides.4-巯基苯硼酸功能化金纳米粒子的简便合成及其用于糖肽的选择性富集。
Rapid Commun Mass Spectrom. 2009 Nov;23(22):3493-500. doi: 10.1002/rcm.4258.

一种用于在探针上检测糖蛋白和糖肽的硼酸功能化板的快速制备方法。

A Rapid Approach for Fabricating Boronic Acid-Functionalized Plates for On-Probe Detection of Glycoprotein and Glycopeptide.

作者信息

Liu Yu-Ching, Chen Chao-Jung

机构信息

Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital.

Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital; Graduate Institute of Integrated Medicine, China Medical University.

出版信息

Mass Spectrom (Tokyo). 2017;6(Spec Iss):S0063. doi: 10.5702/massspectrometry.S0063. Epub 2017 Feb 24.

DOI:10.5702/massspectrometry.S0063
PMID:28337401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5358407/
Abstract

We developed a rapid and simple approach without using complex mechanical or chemical protocols to fabricate boronic acid-functionalized plates for glycoprotein or glycopeptide enrichment and mass spectrometry (MS) analysis. By coating the boronic acid-functionalized silica particles on a polydimethylsiloxane (PDMS)-coated matrix-assisted laser desorption/ionization (MALDI) plate, these particles can form a firmly monolayer of particles on PDMS membrane for sample handling without peeling off. The boronic acid particles-coated PDMS plate (BP plate) was successfully applied to the enrichment of horseradish peroxidase (HRP) protein and their digested glycopeptides.

摘要

我们开发了一种快速且简单的方法，无需使用复杂的机械或化学方案来制备用于糖蛋白或糖肽富集及质谱（MS）分析的硼酸功能化板。通过将硼酸功能化的二氧化硅颗粒涂覆在聚二甲基硅氧烷（PDMS）涂层的基质辅助激光解吸/电离（MALDI）板上，这些颗粒可以在PDMS膜上形成牢固的单层颗粒，用于样品处理而不会脱落。硼酸颗粒涂覆的PDMS板（BP板）已成功应用于辣根过氧化物酶（HRP）蛋白及其消化后的糖肽的富集。