Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai, P R China.
Proteomics. 2010 Mar;10(5):1079-86. doi: 10.1002/pmic.200900097.
Boronic acid functionalized gold-coated Si wafer has been used as MALDI plate to isolate and enrich glycopeptides for MS analysis. This on-plate enrichment strategy offers good benefits due to the combination of specific selectivity through enrichment and direct manipulation on the wafer. First, solution transfer and eluting steps required in conventional enrichment strategies are not needed any more, thereby reducing sample loss during these steps. Second, the LODs of glycopeptides have been increased by two orders of magnitude. Third, non-specific bindings have not been detected even when non-glycopeptides are 100 times more than glycopeptides. Furthermore, the recovery of glycopeptide is up to 65.8% and glycopeptides even can be sensitively detected in the presence of 200 mM ammonium bicarbonate or physiological buffer PBS.
硼酸功能化的金涂覆硅片已被用作 MALDI 板，用于分离和富集糖肽进行 MS 分析。这种板上富集策略具有很好的优势，因为通过富集和在晶片上直接操作相结合，具有特异性选择性。首先，不再需要传统富集策略中所需的溶液转移和洗脱步骤，从而减少了这些步骤中的样品损失。其次，糖肽的检测限提高了两个数量级。第三，即使非糖肽是糖肽的 100 倍，也没有检测到非特异性结合。此外，糖肽的回收率高达 65.8%，即使在存在 200mM 碳酸氢铵或生理缓冲液 PBS 的情况下，也可以灵敏地检测到糖肽。
