Jiang Shiwei, Xu Zhonghua, Zhao Yuanyuan, Yan Lei, Zhou Zunlin, Gu Gangli
Department of Urology, Qilu Hospital, Shandong University, Jinan, China.
Department of Propaganda, Shandong Provincial Traditional Chinese Medical Hospital, Jinan, China.
Biomed Res Int. 2017;2017:1850256. doi: 10.1155/2017/1850256. Epub 2017 Feb 28.
. This study was to evaluate the utility of the compound graft for tubularized urethroplasty by seeding mesothelial cells onto autogenous granulation tissue. . Silastic tubes were implanted subcutaneously in 18 male rabbits, of which nine underwent omentum biopsies simultaneously for in vitro expansion of mesothelial cells. The granulation tissue covering the tubes was harvested 2 weeks after operation. Mesothelial cells were seeded onto and cocultured with the tissue for 7 days. A pendulous urethral segment of 1.5 cm was totally excised. Urethroplasty was performed with mesothelial cell-seeded tissue tubes in an end-to-end fashion in nine rabbits and with unseeded grafts in others as controls. Serial urethrograms were performed at 1, 2, and 6 months postoperatively. Meanwhile, the neourethra was harvested and analyzed grossly and histologically. . Urethrograms showed cell-seeded grafts maintained wide at each time point, while strictures formation was found in unseeded grafts. Histologically, layers of urothelium surrounded by increasingly organized smooth muscles were observed in seeded grafts. In contrast, myofibroblasts accumulation and extensive scarring occurred in unseeded grafts. . Mesothelial cell-seeded granulation tissue tube can be successfully used for tubularized urethroplasty in male rabbits.
本研究旨在通过将间皮细胞接种到自体肉芽组织上来评估复合移植物在管状化尿道成形术中的效用。在18只雄性兔皮下植入硅橡胶管，其中9只同时进行网膜活检以体外扩增间皮细胞。术后2周收集覆盖在管子上的肉芽组织。将间皮细胞接种到该组织上并共同培养7天。完全切除1.5厘米的下垂尿道段。9只兔采用接种间皮细胞的组织管进行端端式尿道成形术，其他兔采用未接种的移植物作为对照。术后1、2和6个月进行系列尿道造影。同时，获取新尿道并进行大体和组织学分析。尿道造影显示，接种细胞的移植物在每个时间点都保持通畅，而未接种的移植物出现狭窄形成。组织学上，在接种的移植物中观察到由逐渐有组织的平滑肌包围的尿路上皮层。相比之下，未接种的移植物中出现肌成纤维细胞积聚和广泛瘢痕形成。接种间皮细胞的肉芽组织管可成功用于雄性兔的管状化尿道成形术。
